Paxillus involutus (Batsch) Fr. + Pinus

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Literature

references
  Giltrap NJ (1979) Experimental studies on the establishment and stability of ectomycorrhizas. Diss Univ Sheffield.
Iingleby K, Masons PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.
Laiho O (1970) Paxillus involutus as a mycorrhizal symbiont of forest trees. Acta For Fenn 106: 1-72.
Mleczko P (1997) Paxillus involutus (Batsch) Fr. + Pinus sylvestris L. Descr Ectomyc 2: 25-70.
Turnau K, Kottke I, Oberwinkler F (1993) Paxillus involutus
Pinus sylvestris mycorrhizae from heavily polluted forest. I. Element localization using electron energy loss spectroscopy and imaging. Bot Acta 106: 213-219.
Turnau K, Kottke I, Dexheimer J (1994) Paxillus involutus/Pinus sylvestris mycorrhizae from heavily polluted forest. II. Ultrastructural and cytochemical observations. Bot Acta 107: 73-80.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
16 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
or    coralloid
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  abundant, dense
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.65 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
or    abundant
exploration type
  long distance
Morphology of the unramified ends
shape
  straight
or    bent
or    sinuous
or    tortuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
or    white
older parts colour
  brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    not smooth
or    glistening
mantle surface {in detail} kind
  loosely woolly
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
or    abundant
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.14 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  concolourous to mantle
or    brownish
connection to mantle kind
  distinct
origin location
  proximal
margin habit
  hairy
Morphology of sclerotia
presence
  present
abundance
  infrequent
or    abundant
shape
  globular
or    elongated
diameter
0.16 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2 mm    Maximum value
colour
  dark brown
or    brown
or    white
formation location
  on the mantle
or    laterally on rhizomorphs

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    cystidia
emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
and    hyphae arranged net-like, with prominent cystidia (type D)
hyphal system kind
  undifferentiated
septa thickness {relative to cell walls}
  as thick as walls
cell shape
  cylindric, not constricted at septa
and    cylindric, constricted at septa
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  {in some cells} homogeneous, brownish
cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
cell length
8 µm    Minimum value
27 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
cell length
6 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
73 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
78 µm    Upper value of unspecified range (could be µ+s.d., but not known)
100 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
inner mantle layer hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
unlayered mantle hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
unlayered mantle hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
108 µm    Maximum value
radially diameter
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
47.7 µm    Mean (= average)
mean shape-ratio TCq
2.9     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
55 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
55 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
27.3 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.75     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  in two rows, not reaching endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two
structure {in plan view}
  of palmetti type
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
backwards-oriented ramifications presence
  present
backwards-oriented clamps presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  fusiform (type F)
or    globular (type G)
septa presence
  absent
septa kind
  absent, with basal clamp only
diameter {proximal}
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
length
13 µm    Minimum value
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  similar in thickness
or    thicker
surface habit
  smooth
contents presence
  absent
contents type
  absent

Anatomy of emanating hyphae
shape
  not striking
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in lateral view}
  as a semicircle
or    less than a semicircle
or    not constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  absent
or    present
abundance
  infrequent
distribution
  not specified

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
or    inflated
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
27 µm    Minimum value
33 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
140 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
nodia presence
  present
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
contents presence-kind
  filled with brownish or yellowish substances
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of sclerotia
internal structure organisation
  pseudoparenchymatous with roundish cells
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
{of foreign origin} presence
  absent
shape
  globular
Ultrastructure
septal pores type
  as dolipores with perforated parenthesome

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
amyloidity haustoria presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sudan III presence
  absent
reaction with sulpho-vanillin presence
  absent
reaction with toluidin blue presence
  absent

Ecology

substrate
  in organically enriched mineral horizon
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant subgenus-section
  two-needle-pines
plant habitat kind
  forests, woods

Fungus

family
  Paxillaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Autofluorescence of mantle in section with UV-filter bluish and with dark yellow hyphal contents, with blue-filter light yellowish-ochre and with dark yellow hyphal contents; mantle cells in brillant-cresyl-blue with bluish walls and cytoplasm, in cotton-blue hyphae inside blue.

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