Paxillus involutus (Batsch) Fr. + Pinus

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Literature

• references
  Giltrap NJ (1979) Experimental studies on the establishment and stability of ectomycorrhizas. Diss Univ Sheffield.
Iingleby K, Masons PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.
Laiho O (1970) Paxillus involutus as a mycorrhizal symbiont of forest trees. Acta For Fenn 106: 1-72.
Mleczko P (1997) Paxillus involutus (Batsch) Fr. + Pinus sylvestris L. Descr Ectomyc 2: 25-70.
Turnau K, Kottke I, Oberwinkler F (1993) Paxillus involutus
Pinus sylvestris mycorrhizae from heavily polluted forest. I. Element localization using electron energy loss spectroscopy and imaging. Bot Acta 106: 213-219.
Turnau K, Kottke I, Dexheimer J (1994) Paxillus involutus/Pinus sylvestris mycorrhizae from heavily polluted forest. II. Ultrastructural and cytochemical observations. Bot Acta 107: 73-80.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
16 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous
or    coralloid
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  abundant, dense
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.65 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
or    abundant
• exploration type
  long distance
Morphology of the unramified ends
• shape
  straight
or    bent
or    sinuous
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
or    white
• older parts colour
  brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    not smooth
or    glistening
• mantle surface {in detail} kind
  loosely woolly
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
or    abundant
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.14 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
or    brownish
• connection to mantle kind
  distinct
• origin location
  proximal
• margin habit
  hairy
Morphology of sclerotia
• presence
  present
• abundance
  infrequent
or    abundant
• shape
  globular
or    elongated
• diameter
0.16 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2 mm    Maximum value
• colour
  dark brown
or    brown
or    white
• formation location
  on the mantle
or    laterally on rhizomorphs

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
and    hyphae arranged net-like, with prominent cystidia (type D)
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, not constricted at septa
and    cylindric, constricted at septa
• cell pigment location-colour
  membranaceously yellowish
• cell contents presence-kind
  {in some cells} homogeneous, brownish
• cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
• cell length
8 µm    Minimum value
27 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
6 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
73 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
78 µm    Upper value of unspecified range (could be µ+s.d., but not known)
100 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• unlayered mantle hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• unlayered mantle hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
108 µm    Maximum value
• radially diameter
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
47.7 µm    Mean (= average)
• mean shape-ratio TCq
2.9     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
55 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
55 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
27.3 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.75     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  in two rows, not reaching endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two
• structure {in plan view}
  of palmetti type
• lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• backwards-oriented ramifications presence
  present
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
• anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  fusiform (type F)
or    globular (type G)
• septa presence
  absent
• septa kind
  absent, with basal clamp only
• diameter {proximal}
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
• length
13 µm    Minimum value
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness {relative to mantle cells}
  similar in thickness
or    thicker
• surface habit
  smooth
• contents presence
  absent
• contents type
  absent

Anatomy of emanating hyphae
• shape
  not striking
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in lateral view}
  as a semicircle
or    less than a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  absent
or    present
• abundance
  infrequent
• distribution
  not specified

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
or    inflated
• anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
27 µm    Minimum value
33 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
140 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• nodia presence
  present
• internal nodia presence
  absent
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  filled with brownish or yellowish substances
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of sclerotia
• internal structure organisation
  pseudoparenchymatous with roundish cells
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• {of foreign origin} presence
  absent
• shape
  globular
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity haustoria presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with sudan III presence
  absent
• reaction with sulpho-vanillin presence
  absent
• reaction with toluidin blue presence
  absent

Ecology

• substrate
  in organically enriched mineral horizon
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Paxillaceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Autofluorescence of mantle in section with UV-filter bluish and with dark yellow hyphal contents, with blue-filter light yellowish-ochre and with dark yellow hyphal contents; mantle cells in brillant-cresyl-blue with bluish walls and cytoplasm, in cotton-blue hyphae inside blue.

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