"Piceirhiza cornuta" + Picea

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Literature

references
  MONTECCHIO L, Agerer R (1997) Piceirhiza cornuta + Picea abies (L.) Karst. Descr Ectomyc 2: 31-35.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
main axis diameter
0.26 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.32 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.84 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.18 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.27 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  ochre, yellowish brown
very tip colour
  ochre, yellowish brown
or    whitish
older parts colour
  brown
or    ochre, yellowish brown
or    red
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
mantle surface {in detail} kind
  loosely short-spiny
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  cystidia
emanating elements cystidia location
  on outer mantle layer
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  aculeate, acuminated horn-shaped
hyphal net cells diameter
4 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
hyphal net cell walls habit
  distinct, rather thick
pores between cells presence
  absent
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  absent
cell pigment location-colour
  membranaceously brownish
cell contents presence-kind
  absent
cell diameter
1.8 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
6.5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
28 µm    Maximum value
cell density
2     Minimum value
4     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
cell wall surface habit
  smooth
and    with few soil particles
cell wall projections presence
  present
cell wall projections abundance
  infrequent
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  pseudoparenchymatous
cell pigment location-colour
  colourless
cell diameter
2.4 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Minimum value
7.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
cell density
2     Minimum value
5     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.3 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
or    pseudoparenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  present
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9.8 µm    Maximum value
cell length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
9.5 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
29.5 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
19.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
29 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36.5 µm    Maximum value
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
2 µm    Minimum value
9.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
28 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Minimum value
3.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae tangentially length
2.4 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
middle mantle layer hyphae radially diameter
1.6 µm    Minimum value
2.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3.8 µm    Minimum value
7.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
1.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
7.5 µm    Minimum value
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
69 µm    Upper value of unspecified range (could be µ+s.d., but not known)
83 µm    Maximum value
radially diameter
4 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
47.8 µm    Mean (= average)
mean shape-ratio TCq
7.2     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
87 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
58.6 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.3     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
or    three
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.7 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two
structure {in plan view}
  infrequently lobed
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
2 µm    Minimum value
9.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
28 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Minimum value
3.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae tangentially length
2.4 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
middle mantle layer hyphae radially diameter
1.6 µm    Minimum value
2.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9.7 µm    Maximum value
inner mantle layer hyphae radially diameter
1.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
shape
  tangentially-oval to tangentially-elliptic
tangentially length
6.5 µm    Minimum value
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
41 µm    Maximum value
radially diameter
4 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangential length TCt
28.4 µm    Mean (= average)
mean shape-ratio TCq
4.6     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
18.5 µm    Minimum value
25.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
37 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells radially diameter
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
27.1 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.7     Mean (= average)

Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
septal pores configuration
  globular thickenings
backwards-oriented ramifications presence
  absent
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  awl-shaped, bristle-like (type A)
ramification presence-position
  absent
septa presence
  present
septa kind
  simple
diameter {proximal}
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
diameter {distal}
2.5 µm    Mean (= average)
length
40 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
160 µm    Maximum value
cell wall colour
  absent
cell wall thickness
0.5 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  thicker
cell wall evenness
  uneven in thickness
surface habit
  smooth
contents presence
  absent
contents type
  absent

Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  approximately 90°
distance {from septum}
  in considerable distance from the septum
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in dorsal view}
  cylindric
clamps outline {in lateral view}
  less than a semicircle
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  abundant
distribution
  evenly distributed

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
80 µm    Minimum value
100 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Minimum value
0.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  uneven in thickness


Anatomy of rhizomorphs
type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
Ultrastructure
mantle matrix presence-kind
  dark

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with brillant-cresyl-blue presence
  present
reaction with FeSO4 presence
  present
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  amyloid
amyloidity or dextrinoidity location
  septa, globular thickenings of septa
reaction with sulpho-vanillin presence
  absent

Ecology

substrate
  in organic layer
{occurence in} humus type
  mull
soil pH
  pH 6 - 7
geographic occurrence continent
  Europe

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Remarks

public notes
  Mycorrhizal ends straight to bent, older parts ochre-yellowish-brown to reddish brown to red; middle mantle layers in plan view with epidermoid cells; plan view of inner mantle layers with epidermoid cells in pseudoparenchymatous parts; cystidia straight or irregularly bent and with a foot cell; emanating hyphae septal distance is taken between clamps, distance between simple septa (25-)30(-42) um; autofluorescence of mantle in section with UV-filter bluish throughout, with blue-filter yellowish ochre throughout, with green-filter slightly reddish throughout; mantle in Melzer's reagent with infrequent amyloid septa and golublar thickenings of septa, in FeSO4 cell walls greenish, in brillant-cresyl-blue cell walls slightly bluish.

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