Tuber brumale Vitt. + Pinus

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Literature

• references
  Fontana A, Fasolo Bonfante P (1971) Sintesi micorrizica di Tuber brumale Vitt. con Pinus nigra Arnold. Allionia 17: 15-18.
Zambonelli A, Salomoni S, Pisi A (1995) Caratterizzazione anatomo-morfologica delle micorrize di Tuber borchii, Tuber aestivum, Tuber mesentericum, Tuber brumale, Tuber melanosporum su Pinus pinea. Mic Ital 1995(2): 119-137.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6.3 mm    Maximum value
• ramification presence-type
  absent
or    dichotomous
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• shape
  straight
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
5.7 mm    Maximum value
• diameter
0.54 mm    Mean (= average)
• colour
  ochre, yellowish brown
or    red
• very tip colour
  ochre, yellowish brown
or    yellowish
• older parts colour
  brown
or    red
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
or    not smooth
• mantle surface {in detail} kind
  densely short-spiny
or    loosely short-spiny
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  cystidia
• emanating elements cystidia location
  on outer mantle layer
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells (type L)
• septa clamps presence
  absent
• cell density
3     Minimum value
3.6     Lower value of unspecified range (could be µ-s.d., but not known)
5.8     Upper value of unspecified range (could be µ+s.d., but not known)
6     Maximum value
• cell wall with globular thickenings
  absent
• cell wall projections presence
  absent
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous
• cell density
5     Minimum value
5.6     Lower value of unspecified range (could be µ-s.d., but not known)
7.6     Upper value of unspecified range (could be µ+s.d., but not known)
8     Maximum value
Anatomy of the inner mantle layer
• organisation
  pseudoparenchymatous
• septa clamps presence
  absent
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
27 µm    Minimum value
32 µm    Lower value of unspecified range (could be µ-s.d., but not known)
61 µm    Upper value of unspecified range (could be µ+s.d., but not known)
68 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
5.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
5.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• radially diameter
24.3 µm    Mean (= average)
• mean tangenial length TCt
48.2 µm    Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
60 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
63.7 µm    Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  in two rows, not reaching endodermis
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3.8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
6.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
6.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Mean (= average)
• radially diameter
22 µm    Mean (= average)
• mean tangential length TCt
51.7 µm    Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
29.5 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
60.4 µm    Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  apparently two rows deep
Anatomy of the emanating elements in general
• backwards-oriented clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  awl-shaped, bristle-like (type A)
• ramification presence-position
  absent
• septa presence
  present
• septa kind
  simple
• diameter {proximal}
3 µm    Minimum value
3.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• diameter {distal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
70 µm    Minimum value
86 µm    Lower value of unspecified range (could be µ-s.d., but not known)
215.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
265 µm    Maximum value
• cell wall colour
  yellowish

Anatomy of emanating hyphae
• shape
  not striking


Anatomy of the septa of emanating hyphae
• clamps presence
  absent
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
3.1 µm    Minimum value
4.4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Tuberaceae
• fruitbodies growth habit
  hypogeous

Remarks

• public notes
  Mycorrhizal ends ochre-red, very tips yellow- ochre, older parts red-brown to brown; middle mantle layers epidermoid; inner mantle layers epidermoid.

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