Acephala macrosclerotiorum Münzenberger & Bubner + Pinus

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Literature

• references
  Münzenberger B, Bubner B, Wöllecke J, Sieber TN, Bauer R, Fladung M, Hüttl RF (2009) The ectomycorrhizal morphotype Pinirhiza sclerotia is formed by Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii. Mycorrhiza 19: 481-492. - Wöllecke J (2001) Charakterisierung der Mykorrhizazönosen zweier Kiefernforste unterschiedlicher Trophie. Cotbuser Schriften Vol 17, Brandenburgische Technische Universität. -

Morphology

Morphology of the mycorrhizal system
• ramification presence-type
  absent
or    dichotomous
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
or    bent
or    sinuous
• shape {of distal end}
  not inflated, cylindric
• colour
  black
• older parts colour
  black
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely grainy or warty
or    loosely woolly
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
Morphology of sclerotia
• presence
  present
• abundance
  abundant
• shape
  cushion-shaped
• diameter
0.1 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  black
• formation location
  on the mantle

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• completeness
  incomplete
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• hyphal net clamps presence
  absent
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously blackish
• cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
• blue granules location
  within cells
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present


Anatomy of the Hartig net in longitudinal section
• presence
  present
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  two
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae clamps presence
  absent
Anatomy of cystidia


Anatomy of emanating hyphae
• cell pigment location-colour
  membranaceously blackish


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of sclerotia
• center structure habit
  compactly filled
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Ultrastructure
• septal pores type
  simple, with Woronin bodies

Autofluorescence

• whole mycorrhizae UV 366 nm colour-presence
  absent

Chemical reactions

• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with KJ presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with toluidin blue presence
  absent

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