"Pinirhiza arachnorosea" + Pinus

– Item listing –

Literature

• references
  Wöllecke J, Münzenberger B, Hüttl RF (1997) "Pinirhiza arachnorosea" + Pinus sylvestris L. Descr Ectomyc 2: 37-42.
Wöllecke J, Münzenberger B, Hüttl RF (1998) Pinirhiza arachnorosea. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 125, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate
or    irregularly pinnate, dichotomous-like
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  nest-like, hyphal mats
• main axis diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.45 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
Morphology of the unramified ends
• shape
  straight
or    bent
or    sinuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  red
or    white
• older parts colour
  brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
• diameter
0.06 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.075 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
or    flat
• colour
  red
• ramification kind-frequency
  repeatedly into smaller filaments
• connection to mantle kind
  oblique
or    fan-like
• origin location
  not specific
• margin habit
  hairy
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  undifferentiated
• hyphae hyphal junctions angle {between}
  ca. 90°
and    ca. 120°
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, constricted at septa
• cell pigment location-colour
  absent
• cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell length
100 µm    Lower value of unspecified range (could be µ-s.d., but not known)
140 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, with broad streaks of parallel hyphae
• cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  present
• cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
5 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
87 µm    Maximum value
• mantle different layers presence
  not discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
26 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
3 µm    Minimum value
5 µm    Mean (= average)
20 µm    Maximum value
• mean tangenial length TCt
43.1 µm    Mean (= average)
• mean shape-ratio TCq
7     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
46.5 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.4     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section
• mantle different layers presence
  not discernible
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in cross-section
• presence
  present
• shape
  round

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
18 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
52 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
31.2 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
Anatomy of the emanating elements in general
• septal pores configuration
  dolipore-like structures
• anastomoses type
  closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
Anatomy of cystidia


Anatomy of emanating hyphae
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°

Anatomy of the septa of emanating hyphae
• clamps presence
  present

Anatomy of secondary septa of emanating hyphae
• presence
  absent

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Minimum value
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
110 µm    Lower value of unspecified range (could be µ-s.d., but not known)
140 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner


Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent

Autofluorescence

• mantle in section UV-filter 340-380 nm presence
  present

Chemical reactions

• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  present
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  present

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Remarks

• public notes
  Mycorrhizal systems irregularly ramified; mycorrhizal ends sinuous to nearly straight, white to reddish and if air displaced by water brown due to root colour, older parts brown due to root colour; rhizomorphs redder than mantle; middle mantle layers in plan view behind the very tip with broad streaks of parallel hyphae; Hartig net in plan view often irregularly shaped; autofluroescence of mantle in section with UV-filter lacking, but rhizomorphs blue and emanating hyphae yellow or lacking; mantle in sulfo-vanillin with slightly red inner layer, in KOH mantle blue to greenish blue, whole mycorrhizae greenish blue, in cotton-blue walls and cytoplasm blue, in ethanol reddish brown.

– Item listing –