Russula pectinatoides Peck + Quercus

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Literature

• references
  Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate [irregularly]
• main axis diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  present
• shape
  straight
or    sinuous [slightly]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brownish
or    ochre, yellowish brown
• very tip colour
  brownish [light]
• after injury colour
  unchanged
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    covered with soil particles
or    not smooth
• mantle surface {in detail} kind
  densely short-spiny
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
or    cystidia
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
• matrix kind
  gelatinous
• hyphae sticky matrix on hyphal surface presence
  present
• septa clamps presence
  absent
• cell pigment location-colour
  absent
and    membranaceously yellowish
• cell contents presence-kind
  absent
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous
or    pseudoparenchymatous
• cell shape
  variable
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [densely]
• hyphae arrangement
  without pattern [often in parallel]
• hyphae vessel-like presence
  absent
• septa clamps presence
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous
Anatomy of the mantle in longitudinal section



Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae clamps presence
  absent
• anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  flask-shaped with an apical knob (type D)
• apical knobs number
1     Mean (= average)
• apical knobs arrangement
  solitary, not in chains
or    symmetrically
• apical knobs shape
  globose [to subglobose]
• apical knobs diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
• apical knobs cell wall thickness {relative to cystidial body}
  thicker than those of cystidial body
• septa presence
  absent
• foot cell presence
  absent
• diameter {proximal}
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter {distal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
or    yellowish
• cell wall thickness
0.3 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
• surface habit
  smooth
• contents presence
  present
• contents type
  brownish, with latex-like droplets

Anatomy of emanating hyphae
• drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    with a gelatinous envelope
• anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Mean (= average)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent

Chemical reactions

• reaction with cotton-blue-lactic-acid presence
  present [cell walls blue]
• reaction with FeSO4 presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  present [contents of some cystidia dark brown]

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Fagaceae
• plant genus
  Quercus
• plant habitat kind
  gardens, parks

Fungus

• family
  Russulaceae
• subgenus-section
  Russula sect. Ingratae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
335     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
195     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
150     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
340     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
335     Mean (= average)

Remarks

• public notes
  EcoR I: 360 bp, 320 bp; Taq I: 280 bp, 160 bp, 105 bp, 60 bp

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