Russula alduterina (Fr.) Peck + Picea

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Literature

references
  Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
11 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  absent
or    monopodial-pinnate
or    monopodial-pyramidal
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  contact
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown [redbrown]
very tip colour
  brownish [light redbrown]
after injury colour
  unchanged
mantle {distinct} surface visibility
  present
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
matrix presence
  present
matrix location
  surface net
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  some cells containing droplets, staining in sulpho-vanillin; shape variable (type N)
and    epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  with roundish lobes
hyphal net cells diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
hyphal net cell walls habit
  faint, delicate, inconspicuous, rather thin
and    distinct, rather thick
hyphal net clamps presence
  absent
clavate end cell cover presence
  absent
matrix kind
  gelatinous
hyphae arrangement
  not specific
septa clamps presence
  absent
cell pigment location-colour
  absent
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
cell wall projections presence
  present
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  pseudoparenchymatous
matrix kind
  not applicable
hyphae arrangement
  plectenchymatous, without pattern
cell shape
  epidermoid
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
Anatomy of the inner mantle layer
organisation
  pseudoparenchymatous
matrix kind
  not applicable
hyphae arrangement
  without pattern
cell shape
  epidermoid
hyphae vessel-like presence
  absent
septa clamps presence
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  with droplets, that stain in sulpho-vanillin
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section



Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae clamps presence
  absent
Anatomy of cystidia


Anatomy of emanating hyphae
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present [cell walls blue]
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [contents of gloeoplerous cells grey to black]

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Russula sect. Insidiosinae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
440     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
240     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
380     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
320     Mean (= average)

Remarks

public notes
  EcoR I: 400 bp, 300 bp; Taq I: 380 bp, 200 bp, 80 bp, 75 bp, 60 bp

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