Laccaria amethystina (Bolt.: Hooker) Murr. + Fagus

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Literature

• references
  Brand F (1988) Laccaria amethystina. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 18, Einhorn-Verlag, Schwäbisch Gmünd.
Brand F, Agerer R (1986) Studien an Ektomykorrhizen VIII. Die Mykorrhizen von Lactarius subdulcis, Lactarius vellereus und Laccaria amethystina an Buche. Z Mykol 52: 287-320.
Meyer FH (1963) Laccaria amethystina (Bolt. ex Fr.) Berk. et Br., ein zur Mykorrhizabildung an der Buche befähigter Pilz. Ber Dtsch Bot Ges 76: 90-96.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.55 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  medium distance smooth
Morphology of the unramified ends
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
or    grey
• very tip colour
  violet, dark reddish blue
• older parts colour
  brown
or    ochre, yellowish brown
or    grey
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely cottony
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  concentrated proximally
Morphology of rhizomorphs
• cross-section shape
  round or roundish
• colour
  violet, dark reddish blue
or    grey
or    whitish
• ramification kind-frequency
  infrequently, at restricted points
• origin location
  proximal
• margin habit
  hairy
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  with rather short, obtuse, even finger-like branches
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, not constricted at septa
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell diameter
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  absent


Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  periepidermal
or    in two rows, not reaching endodermis
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.8 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  absent


Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  apparently two rows deep
or    apparently one and a half row deep
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
• lobes width
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  thinner
or    as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
or    as broad as
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  absent

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
200 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)


Anatomy of rhizomorphs
• type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• internal nodia presence
  absent
• gelatinized hyphae presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  present
• reaction with FeSO4 presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  present
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  present
• reaction with ruthenium-red presence
  absent
• reaction with sudan III presence
  absent
• reaction with sulpho-vanillin presence
  absent
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Fagaceae
• plant genus
  Fagus
• plant habitat kind
  forests, woods

Fungus

• family
  Tricholomataceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Autofluorescence of mantle in section with UV-filter inclusive emanating hyphae light blue, with blue-filter inclusive emanating hyphae greenish orange; mantle in KOH pigment not bleaching rather becoming more intense, in lactic acid violet pigment bleaching and at first becoming vinaceous, in brillant-cresyl-blue walls violet, in toluidin-blue blue-violet, in cotton-blue walls bluish, in ethanol violet pigment bleaching, in phenole violet pigment bleaching, in chlorazol-black blue-grey.

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