Cortinarius tucumanensis Moser + Alnus

– Item listing –

Literature

references
  Becerra AG, Beenken L, Pritsch K, Daniele G, Schloter M, Agerer R (2005). Morphological description and molecular characterization of Lactarius aff. omphaliiformis, Russula alnijorullensis and Cortinarius tucumanensis ectomycorrhizas on Alnus acuminata. Mycologia 97:1047-1057

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  absent
or    monopodial-pinnate [rarely]
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  solitary or in small numbers
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
exploration type
  medium distance fringe
Morphology of the unramified ends
mantle hydrophobicity presence
  present
shape
  sinuous
or    tortuous
shape {of distal end}
  not inflated, cylindric
or    tapering
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
9 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  white
older parts colour
  ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    not smooth
mantle surface {in detail} kind
  densely cottony
or    loosely cottony
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  concolourous to mantle
or    white
after injury colour
  unchanged
ramification kind-frequency
  repeatedly into smaller filaments
connection to mantle kind
  fan-like
origin location
  not specific
connection to substrate
  growing into the soil or organic layers
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal net cells shape
  not applicable
hyphal net cells diameter
  not applicable
hyphal net cells length
  not applicable
hyphal net cell walls habit
  not applicable
hyphal net cell walls thickness
  not applicable
hyphal net clamps presence
  not applicable
clavate end cell cover presence
  absent
hyphal system kind
  undifferentiated
hyphae short protrusions presence
  absent
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  present
septa clamps abundance
  abundant
cell shape
  cylindric, not constricted at septa
and    cylindric, constricted at septa
cell pigment location-colour
  absent
cell contents presence-kind
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
Anatomy of the middle mantle layer
presence
  absent
Anatomy of the inner mantle layer
organisation
  plectenchymatous with pseudoparenchymatous nests of cells
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
15 µm    Maximum value
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
62 µm    Lower value of unspecified range (could be µ-s.d., but not known)
160 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous [loosely]
outer mantle layer hyphae tangentially length
23 µm    Lower value of unspecified range (could be µ-s.d., but not known)
78 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer presence
  absent
inner mantle layer organisation
  pseudoparenchymatous
inner mantle layer hyphae tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    round
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  periepidermal
or    paraepidermal [rarely]
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two
structure {in plan view}
  of palmetti type
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
backwards-oriented clamps presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses surface habit
  smooth
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  straight
or    wavy
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps surface kind
  smooth
clamps width {relative to hypha in lateral view}
  thinner than

Anatomy of secondary septa of emanating hyphae
presence
  absent

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
or    even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
3.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of the warts occouring on emanating hyphae
presence
  absent
Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  present, not specified
roundish plasmatically yellow cells presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  present, not specified
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  present, not specified
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  present, not specified
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  present, not specified
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present [emanating hyphe blue]
reaction with ethanol 70% presence
  absent
reaction with KOH 10% presence
  present [whole mycorrhiza dark]
reaction with lactic acid presence
  present [orange]
reaction with Melzer's reagent presence
  absent
reaction with NH4OH
  absent [tips of whole mycorrhiza black]
reaction with sulpho-vanillin presence
  absent

Ecology

geographic occurrence continent
  South America

Tree

plant family
  Betulaceae
plant genus
  Alnus
plant habitat kind
  forests, woods

Fungus

family
  Cortinariaceae

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
290     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
190     Mean (= average)

Remarks

public notes
  TaqI: 320/240; EcoRI: 330/270

– Item listing –