Pinirhiza multifurcata + Pinus

– Item listing –

Literature

• references
  Wei J, Agerer R (2011) Two sebacinoid ectomycorrhizae on Chinese pine. Mycorrhiza 21: 105-115

Morphology

Morphology of the mycorrhizal system
• ramification presence-type
  absent
or    dichotomous
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
• abundance
  solitary or in small numbers
• main axis diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
or    bent [sometimes]
• shape {of distal end}
  not inflated, cylindric
• length
0.9 mm    Minimum value
2.5 mm    Mean (= average)
3.8 mm    Maximum value
• diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown [grayish orange-brown]
• very tip colour
  whitish
• older parts colour
  black
or    dark brown
• mantle {distinct} surface visibility
  present
• mantle transparency
  semi-transparent, opaque
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely woolly [hairy]
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  gelatinous matrix between the hyphae (type C)
and    hyphae arranged net-like, repeatedly and squarrosely branched (type E)
• incomplete septa presence
  present
• hyphal net cells length
  not applicable
• hyphal net cell walls thickness
  not applicable
• clavate end cell cover presence
  absent
• matrix kind
  gelatinous
• hyphae short protrusions presence
  absent
• hyphae sticky matrix on hyphal surface presence
  present
• pores between cells presence
  present [in few hyphae septa with a large pore]
• hyphae hyphal junctions angle {between}
  ca. 90°
and    ca. 120°
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell contents presence-kind
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  present
• roundish plasmatically yellow cells presence
  present
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  variable [areas with longer hypahe intermixed with few lobed, multiply branched ones]
• incomplete septa presence
  present
• cell pigment location-colour
  colourless
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell wall thickness
0.3 µm    Mean (= average)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  variable
or    ring-like
• hyphae vessel-like presence
  absent
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  not discernable
• unlayered mantle organization
  plectenchymatous

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic

Anatomy of the Hartig net in longitudinal section
• presence
  present
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  awl-shaped, bristle-like (type A)
or    ramified (type E)
• ramification presence-position
  medial
or    distal
• ramification type
  bifurcate
or    trifurcate
• branches ramification presence
  present
• septa presence
  present [vry infrequent]
• septa kind
  simple
• basal clamp walls abruptly thick
  absent
• foot cell presence
  absent
• diameter {proximal}
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• diameter {distal}
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
• cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall evenness
  even in thickness
• surface habit
  smooth
• contents presence
  absent

Anatomy of emanating hyphae
• shape
  wavy
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
or    Y-shaped
• side-branches at septum number
  one side-branch at septum
or    two true (at one height) side-branches at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  as thick as main part of branch [even somewhat thicker]

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
or    with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell diameter
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Chemical reactions

• reaction with FeSO4 presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  dextrinoid
• amyloidity or dextrinoidity location
  emanating hyphae
or    cystidia

Ecology

• geographic occurrence continent
  Asia

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Sebacinaceae

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