Lactarius picinus Fr. + Picea

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– Enlarged view –

Literature

• references
  Agerer R (1986) Studies on ectomycorrhizae III. Mycorrhizae formed by four fungi in the genera Lactarius and Russula on spruce. Mycotaxon 27: 1-59.
Agerer R (1987) Lactarius picinus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 4, Einhorn-Verlag, Schwäbisch Gmünd.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest. A microscopical atlas. Kernforschungszentrum Karlsruhe.

Morphology

Morphology of the mycorrhizal system
• ramification presence-type
  monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  abundant, dense
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  medium distance smooth
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
• diameter
0.35 mm    Minimum value
0.42 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.45 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.6 mm    Maximum value
• colour
  white
• very tip colour
  whitish
• older parts colour
  brown
or    ochre, yellowish brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
• emanating hyphae presence
  absent
Morphology of rhizomorphs
• cross-section shape
  round or roundish
• colour
  whitish
• ramification kind-frequency
  infrequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  not specific
or    proximal
• margin habit
  smooth
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
Anatomy of laticifers
• presence
  present
• location
  middle mantle layer
or    inner mantle layer
• shape
  straight and even
• cell diameter
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hymeniform, stout and often slightly curved hyphal end-cells, filled with oily droplets which stain in sulpho-vanillin (type I)
• septa clamps presence
  absent
• cell pigment location-colour
  absent
and    plasmatically yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• middle mantle layer hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
30 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
• radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
100 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value

Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• middle mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
• radially diameter
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Mean (= average)
• anatomy emanating elements emanating hyphae cell wall surface habit
  without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs
• type
  differentiated; thick hyphae forming a core, septa complete or sometimes enlarged
(type E)
• internal nodia presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
6     Maximum value
Anatomy of siderophilous granulations
• presence
  absent
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome
• mantle matrix presence-kind
  dark

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  present
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with FeSO4 presence
  present
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with sulpho-vanillin presence
  present
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Lactarius sect. Plinthogali
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Autofluorescence of mantle in section with UV-filter laticifers distinctly blue and remaining hyphae slightly blue-green, with blue-filter laticifers distinctly green and remaining hyphae slightly green; mantle in Melzer's reagent laticifers distinctly brownish (whether contents?), in sulfo-vanillin light red and latificers dark carmine red, in FeSO4 greenish brown, in brillant-cresyl-blue bluish-green and laticifers green, in toluidin-blue violet blue, in cotton-blue bluish, in acid fuchsin slightly pink and laticifers intensely pink, in a-naphthol no reaction, in Kongo-red brownish red, in NH4OH no reaction, in chlorazol-black greyish blue and lacticifers more intensely, in erythrosin pinkish brown and laticifers more intensely, in fast-green green and laticifers dark green.

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