"Populirhiza pustulosa" + Populus

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Literature

• references
  Mleczko P (1997) "Populirhiza pustulosa" + Populus tremula L. Descr Ectomyc 2: 61-66.
Mleczko P (1998) "Populirhiza pustulosa". In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 131, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
• length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6 mm    Maximum value
• ramification presence-type
  monopodial-pinnate
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
• main axis diameter
0.18 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 mm    Maximum value
• diameter
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
• older parts colour
  brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.075 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
• ramification kind-frequency
  frequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  not specific
or    proximal
• margin habit
  hairy
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  occasional patches of roundish cells on the mantle (type F)
• cell shape
  cylindric, not constricted at septa
and    slightly inflated in middle portions
• cell pigment location-colour
  membranaceously yellowish
and    plasmatically brownish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.6 µm    Maximum value
• cell length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
75 µm    Upper value of unspecified range (could be µ+s.d., but not known)
90 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  rough
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• cell wall surface habit
  rough
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  present
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6.5 µm    Maximum value
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
• outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
49 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
13.5 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.42     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  periepidermal
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• backwards-oriented ramifications presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  thinner
or    as thick as
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  fusiform (type F)
• septa presence
  absent
• septa kind
  absent, with basal clamp only
• diameter {proximal}
5 µm    Minimum value
7.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
16 µm    Maximum value
• length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  yellowish
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
• cell wall thickness {relative to mantle cells}
  thicker
• cell wall evenness
  even in thickness
• surface habit
  warty

Anatomy of emanating hyphae
• cell pigment location-colour
  membranaceously yellowish


Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in dorsal view}
  cylindric
• clamps width {relative to hypha in dorsal view}
  thinner than
• clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  absent

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
or    inflated at middle parts
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
95 µm    Upper value of unspecified range (could be µ+s.d., but not known)
105 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)


Anatomy of rhizomorphs
• type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with brillant-cresyl-blue presence
  present
• reaction with FeSO4 presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  absent
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Salicaceae
• plant habitat kind
  forests, woods

Remarks

• public notes
  Mycorrhizal systems irregular monopodial-pinnate; mycorrhizal ends usually bent and infrequently straight, dark ochre, distinctly brownish after displacing air between mantle hyphae by water; inner mantle layers in plan view with weakly developed ring-like structures; cystidial walls only at distal parts thickened; rhizomorph peripheral hyphae club-shaped or elongated, 4.5-10(-15)x4.5-15(-23) um, almost always with yellow, up to 2(-2.5) um thick walls, walls may be gelatinized, swellings usually filled with yellowish brown contents in form of irregular granula; autofluorescence of mantle in section with UV-filter outer layer weakly ochre-orange and inner mantle whitish orange, with blue-filter outer mantle weakly ochre-orange and inner mantle orange-yellow; mantle hyphae in brillant-cresyl-blue with blue walls and inside blue, in toluidin-blue walls blue and hyphal inside violet blue, thick walls of outer mantle hyphae inclusive of warts slightly reddish violet, in safranine hyphae inside reddish.

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