"Populirhiza pustulosa" + Populus

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Literature

references
  Mleczko P (1997) "Populirhiza pustulosa" + Populus tremula L. Descr Ectomyc 2: 61-66.
Mleczko P (1998) "Populirhiza pustulosa". In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 131, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6 mm    Maximum value
ramification presence-type
  monopodial-pinnate
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
main axis diameter
0.18 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 mm    Maximum value
diameter
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
older parts colour
  brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    smooth
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.075 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  concolourous to mantle
ramification kind-frequency
  frequently, at restricted points
connection to mantle kind
  distinct
origin location
  not specific
or    proximal
margin habit
  hairy
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    cystidia
emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  occasional patches of roundish cells on the mantle (type F)
cell shape
  cylindric, not constricted at septa
and    slightly inflated in middle portions
cell pigment location-colour
  membranaceously yellowish
and    plasmatically brownish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.6 µm    Maximum value
cell length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
75 µm    Upper value of unspecified range (could be µ+s.d., but not known)
90 µm    Maximum value
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  rough
Anatomy of the middle mantle layer
organisation
  plectenchymatous
cell wall surface habit
  rough
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  ring-like
septa clamps presence
  present
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6.5 µm    Maximum value
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
49 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
13.5 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.42     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  periepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
backwards-oriented ramifications presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  thinner
or    as thick as
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  fusiform (type F)
septa presence
  absent
septa kind
  absent, with basal clamp only
diameter {proximal}
5 µm    Minimum value
7.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
16 µm    Maximum value
length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  yellowish
cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
cell wall thickness {relative to mantle cells}
  thicker
cell wall evenness
  even in thickness
surface habit
  warty

Anatomy of emanating hyphae
cell pigment location-colour
  membranaceously yellowish


Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in dorsal view}
  cylindric
clamps width {relative to hypha in dorsal view}
  thinner than
clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  absent

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
or    inflated at middle parts
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
95 µm    Upper value of unspecified range (could be µ+s.d., but not known)
105 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)


Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with brillant-cresyl-blue presence
  present
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  absent
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Salicaceae
plant habitat kind
  forests, woods

Remarks

public notes
  Mycorrhizal systems irregular monopodial-pinnate; mycorrhizal ends usually bent and infrequently straight, dark ochre, distinctly brownish after displacing air between mantle hyphae by water; inner mantle layers in plan view with weakly developed ring-like structures; cystidial walls only at distal parts thickened; rhizomorph peripheral hyphae club-shaped or elongated, 4.5-10(-15)x4.5-15(-23) um, almost always with yellow, up to 2(-2.5) um thick walls, walls may be gelatinized, swellings usually filled with yellowish brown contents in form of irregular granula; autofluorescence of mantle in section with UV-filter outer layer weakly ochre-orange and inner mantle whitish orange, with blue-filter outer mantle weakly ochre-orange and inner mantle orange-yellow; mantle hyphae in brillant-cresyl-blue with blue walls and inside blue, in toluidin-blue walls blue and hyphal inside violet blue, thick walls of outer mantle hyphae inclusive of warts slightly reddish violet, in safranine hyphae inside reddish.

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