Cenococcum geophilum Fr. + Picea

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– Enlarged view –

Literature

• references
  Agerer R, Gronbach E (1988) Cenococcum geohilum. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 11, Einhorn-Verlag, Schwäbisch Gmünd.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Bücking E (1979) Fichten-Mykorrhizen auf Standorten der Schwäbischen Alb und ihre Bezsiehung zum Befall durch Fomes annosus. Eur J For Pathol 9: 19-35.
Danielson RM, Pruden M (1989) The ectomycorrhizal status of urban spruce. Mycologia 81(3): 335.341.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibl Mycol 125: 1-216.
Harniman SMK, Durall DM (1996) Cenococcum Fr. + Picea engelmannii (Perry) Engelm. In Goodman DM, Durall DM, Trofymow JA, Berch SM (eds) Concise descriptions of North American ectomycorrhizae. Mycologue Publications, and Canada
B. C. Forest Resource Development Agreement, Canadian Forest Service, Victoria, B. C., pp CDE10.1-10.4.
Ingleby K, Mason PA, Last FT, Fleming LV(1990)
Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.
Weiss M (1988) Ektomykorrhizen von Picea abies. Synthese, Ontogenie und Reaktion auf Umweltschadstoffe. Diss Univ München.

Morphology

Morphology of the mycorrhizal system
• length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
• main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  black
• very tip colour
  black
• older parts colour
  black
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  shiny
• mantle surface {in detail} kind
  densely grainy or warty
or    densely woolly
or    loosely woolly
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
or    abundant
• emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
• presence
  present
• abundance
  infrequent
or    abundant
• shape
  globular
or    elongated
• diameter
0.25 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
• colour
  black
• formation location
  on single hyphae or in the soil

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae star-like arranged and tightly glued together (type G)
• hyphae hyphal junctions angle {between}
  ca. 45° and less
• septa thickness {relative to cell walls}
  thinner than walls
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously brownish
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• cell length
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• cell wall thickness
0.2 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, star-like
• cell pigment location-colour
  colourless
• cell diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
• cell length
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell pigment location-colour
  absent
or    membranaceously yellowish
• cell diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• cell length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
90 µm    Maximum value
• radially diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• mean tangenial length TCt
58.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
5     Lower value of unspecified range (could be µ-s.d., but not known)
5.8     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
99 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
59.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
73.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
2.5     Lower value of unspecified range (could be µ-s.d., but not known)
3.9     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• structure {in plan view}
  of palmetti type
• lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• mean tangential length TCt
32.8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
3.5     Lower value of unspecified range (could be µ-s.d., but not known)
4.1     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
31.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.3     Lower value of unspecified range (could be µ-s.d., but not known)
1.6     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
or    three
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• septal pores configuration
  Woronin bodies
• anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a simple septum, with a short bridge or bridge almost lacking (contact-septum)
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  straight
or    wavy
• cell pigment location-colour
  membranaceously brownish
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
3 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of the warts occouring on emanating hyphae
• presence
  present
• length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of sclerotia
• internal structure organisation
  pseudoparenchymatous with roundish cells
• center structure habit
  compactly filled
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• shape
  as normal hyphae
or    globular
Anatomy of nuclei
• number {per cell}
1     Lower value of unspecified range (could be µ-s.d., but not known)
4     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  round
• diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  absent
• mantle in section blue-filter, 450-490 nm presence
  absent
• mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

• reaction with acid fuchsin presence
  absent
or    present
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent

Ecology

• geographic occurrence continent
  Europe
or    North America

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  nursery
or    forests, woods

Remarks

• public notes
  Mantle hyphae in cotton-blue with greenish-brown walls, in acid fuchsin walls reddish-brown.

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