Lactarius salmonicolor R. Heim & Leclair + Abies

– Item listing –

Literature

• references
  Pillukat A (1996) Lactarius salmonicolor R. Heim & Leclair + Abies alba Mill. Descr Ectomyc 1: 59-64.
Pillukat A (1998) Lactarius salmonicolor. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 122, Einhorn-Verlag, Schwäbisch Gmünd.
Eberhardt U, Verbeken A, Rinaldi AC, Pacioni G, Comandini O (2000) Lactarius ectomycorrhizae on Abies alba: morphological description, molecular characterization, and taxonomic remarks. Mycologia 92(5): 860-873. [Eberhardt et al. (2000): laticifers of more homogeneous diameter (5-9 µm) and with rather thin walls; mantle only 30-40 µm thick; otherwise no evident differences to Pillukat (1996)]

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
19 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• tips {per 10 mm} number
5     Lower value of unspecified range (could be µ-s.d., but not known)
14     Upper value of unspecified range (could be µ+s.d., but not known)
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  abundant, dense
• main axis diameter
0.45 mm    Minimum value
0.6 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
1 mm    Maximum value
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
Morphology of the unramified ends
• shape
  straight
or    bent
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.4 mm    Minimum value
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.55 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.6 mm    Maximum value
• colour
  brown
or    orange
• very tip colour
  yellowish
or    orange
or    whitish
• older parts colour
  brown
or    green
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  present
• mantle secreted latex {after scratching mantle} presence
  present
• mantle secreted latex {after scratching mantle} colour-habit
  orange
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  shiny
or    smooth
• emanating hyphae presence
  absent
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
• ramification kind-frequency
  frequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  not specific
or    proximal
• margin habit
  smooth
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  present
• location
  outer mantle layer
or    middle mantle layer
or    inner mantle layer
• shape
  straight and even
• ramification presence-abundance
  infrequent
• cell diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  gelatinous matrix between the hyphae (type C)
• matrix kind
  gelatinous
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell shape
  cylindric, not constricted at septa
and    cylindric, constricted at septa
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell contents presence-kind
  absent
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4.5 µm    Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
80 µm    Mean (= average)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
75     Lower value of unspecified range (could be µ-s.d., but not known)
120     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• radially diameter
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
11 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
or    in two rows, not reaching endodermis
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
Anatomy of cystidia


Anatomy of emanating hyphae


Anatomy of the septa of emanating hyphae
• clamps presence
  absent




Anatomy of rhizomorphs
• type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  filled with brownish or yellowish substances
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  present
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with sudan III presence
  absent
• reaction with sulpho-vanillin presence
  present
• reaction with toluidin blue presence
  absent

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Abies
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Lactarius sect. Dapetes
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mycorrhizal ends slightly bent and in dense clusters even tortuous, light orange to copper-brown, very tips whitish to yellowish orange, older parts green to brown-olive, whole mycorrhiza turning dark green with age; rhizomorphs transparent when young, older ones coloured like mantle, turning green with age; outer mantle layers in plan view with short protrusions of hyphae; laticifers with 0.2-1.8 um thick cell walls; inner mantle layers in plan view with slightly agglutinated hyphae; rhizomorph peripheral hyphae with short protrusions; in longitudinal section 2-4 of 4-5 layers of cortical cells with Hartig net; autofluorescence of mantle in section with UV-filter slightly bluish and laticifers brighter blue, with blue-filter slightly yellowish and laticifers brighter yellow, with green-filter slightly reddish and laticifers brighter red; mantle in sulfo-vanillin with black laticifer contents, in lactic acid contents of laticifers may form light-reflecting crystals, in water mantle of very fresh and young mycorrhizae may stain grey, reaction reversible with lactic acid.

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