"Piceirhiza harenosa" + Picea

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– Enlarged view –

Literature

• references
  BERG B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.

Morphology

Morphology of the mycorrhizal system
• length
5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
20 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• main axis diameter
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
Morphology of the unramified ends
• shape
  straight
or    bent
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  green
or    grey
• very tip colour
  whitish
• older parts colour
  grey
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
or    not smooth
• mantle surface {in detail} kind
  loosely woolly
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
• emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  undifferentiated
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell length
2 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  with many soil particles
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  colourless
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• cell length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
18 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mantle different layers presence
  not discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae tangentially length
1 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
• unlayered mantle hyphae radially diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
32 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
90 µm    Maximum value
• radially diameter
5 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangenial length TCt
63 µm    Mean (= average)
• mean shape-ratio TCq
4.6     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
14 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
85 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
52 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
62.5 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
2.1     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
Anatomy of the mantle in cross-section
• mantle different layers presence
  not discernible
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae tangentially length
1 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• unlayered mantle hyphae radially diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
0     Minimum value
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
18 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• radially diameter
5 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangential length TCt
29.8 µm    Mean (= average)
• mean shape-ratio TCq
2.2     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
20 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
15 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
52 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
30.7 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  Y-shaped
• distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  infrequent
• distribution
  not specified

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
or    with soil particles
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• shape
  as normal hyphae
or    globular
Anatomy of nuclei
• number {per cell}
1     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  round
• diameter
0.9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
0.9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent
• reaction with sulpho-vanillin presence
  absent

Ecology

• {occurence in} humus type
  mull
• soil pH
  pH 5 - 6
• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Remarks

• public notes
  Mycorrhizal systems irregularly monopodial-pyramidal; mycorrhizal ends sometimes slightly bent, grey to olive-grey, very tips whitish to olive-grey, older parts dark grey; plan view of inner mantle layers sometimes with labyrinthine-like pattern; autofluorescence of mantle in section with UV-filter whitish yellowish, with blue-filter yellow, with green-filter red; when 3 nuclei present, then mostly in one group.

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