Pinirhiza globulifera + Pinus

– Item listing –

Literature

• references
  Mleczko P (1998) "Pinirhiza globulifera" + Pinus sylvestris L. Descr Ectomyc 3: 61-66.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs presence
  absent
• rhizomorphs frequency
  not applicable
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
or    present
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  reddish [pinkish white]
or    lilac, light reddish blue [pinkish white]
• very tip colour
  unknown
• older parts colour
  brownish [with reddish tint]
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
• mantle surface {in detail} kind
  densely short-spiny
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
• matrix kind
  not gelatinous
• hyphae short protrusions presence
  absent
• hyphae sticky matrix on hyphal surface presence
  absent
• hyphae hyphal junctions angle {between}
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  present
• septa clamps abundance
  abundant
• cell shape
  unknown
• cell pigment location-colour
  absent
• cell contents presence-kind
  oily droplets, which do not stain in sulpho-vanillin
• cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
57 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
  unknown
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  plectenchymatous, ring-like
• cell pigment location-colour
  colourless
• cell diameter
  unknown
• cell length
  unknown
• cell contents presence-kind
  oily droplets, which do not stain in sulpho-vanillin
• cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  without pattern
• septa thickness
  unknown
• septa clamps presence
  unknown
• cell pigment location-colour
  absent
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
  unknown
• cell contents presence-kind
  with oily droplets, which do not stain in sulpho-vanillin
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  not discernable
• unlayered mantle organization
  plectenchymatous
• unlayered mantle hyphae tangentially length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• unlayered mantle hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [elongated and constricted at the ends]
• tangentially length
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
57 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
30.5 µm    Mean (= average)
• mean shape-ratio TCq
2.4     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic [also cylindrical]
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
12 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
75 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
57 µm    Upper value of unspecified range (could be µ+s.d., but not known)
90 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
30.5 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.9     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
or    in two rows, not reaching endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two [exceptional]
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two [exceptional]
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  unknown
• intrahyphal hyphae clamps presence
  unknown
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  absent
• backwards-oriented clamps presence
  absent
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses surface habit
  smooth
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  unknown
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  capitate (type N)
• branches ramification presence
  absent
• septa presence
  present
• septa kind
  simple
• septa distance {between}
  unknown
• septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
• basal clamp walls abruptly thick
  absent
• foot cell presence
  absent
• diameter {proximal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter {distal}
4 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• length
28 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  similar
• cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
• cell wall thickness {relative to mantle cells}
  similar in thickness
• cell wall evenness
  even in thickness
• surface habit
  smooth
• contents presence
  present
• contents type
  of colourless oil droplets (=guttulae)

Anatomy of emanating hyphae
• shape
  unknown
• cell pigment location-colour
  absent
• cell contents presence-kind
  oily droplets, which do not stain in sulpho-vanillin
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  unknown
• kind
  unknown
• distance {from septum}
  unknown
• side-branches at septum number
  unknown
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps surface kind
  smooth
• clamps outline {in dorsal view}
  unknown
• clamps width {relative to hypha in dorsal view}
  unknown
• clamps outline {in lateral view}
  unknown
• clamps width {relative to hypha in lateral view}
  unknown
• clamps hole presence
  unknown
• clamps blister-like structure {at basis} presence
  unknown

Anatomy of secondary septa of emanating hyphae
• presence
  unknown
• abundance
  unknown
• distribution
  unknown

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  unknown
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
85 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall layers number
  uniform
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  unknown
• {in foreign ectomycorrhizae} abundance
  unknown
• {in foreign ectomycorrhizae} formed in fungal genus
  unknown
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [mantle blue, oily droplets inside hyhae whitish blue]
• mantle in section blue-filter, 450-490 nm presence
  present [mantle and oily droplets inside hyphae greenish yellow]
• mantle in section green-filter, 530-560 nm presence
  unknown

Chemical reactions

• reaction with acetic acid presence
  unknown
• reaction with acid fuchsin presence
  unknown
• reaction with AgNO3 presence
  unknown
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  unknown
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FEA presence
  unknown
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with HCl presence
  unknown
• reaction with KJ presence
  unknown
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  present [oily droplets dissolved]
• reaction with Melzer's reagent presence
  absent
• reaction with methylene blue presence
  unknown
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  unknown
• reaction with sudan III presence
  present [oily droplets inside hyphae and cystidia reddish]
• reaction with sudan IV presence
  unknown
• reaction with sulpho-vanillin presence
  absent
• reaction with sulphuric acid presence
  unknown
• reaction with toluidin blue presence
  unknown
• reaction with trypan blue presence
  unknown
• reaction with water presence
  unknown

Ecology

• substrate
  in organic layer
• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

– Item listing –