Balsamia alba Harkness + Pinus

– Item listing –

Literature

• references
  Palfner G, Agerer R (1998) Balsamia alba Harkness + Pinus jeffreyi Grev. & Balf. Descr Ectomyc 3: 1-6.

Morphology

Morphology of the mycorrhizal system
• length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.7 mm    Maximum value
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  contact
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
or    tapering [to slightly tapering]
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
• colour
  brown [reddish brown]
• very tip colour
  whitish
• after injury colour
  unknown
• older parts colour
  dark brown
• mantle cortical cells visibility
  visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  shiny
or    smooth
• mantle surface {in detail} kind
  not applicable
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
• emanating elements cystidia location
  not applicable
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  incomplete
• matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• clavate end cell cover presence
  absent
• hyphal system kind
  undifferentiated
• hyphae short protrusions presence
  present
• hyphae sticky matrix on hyphal surface presence
  unknown
• hyphae hyphal junctions angle {between}
  ca. 45° and less
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  absent
• cell shape
  cylindric, constricted at septa
and    completely irregularly shaped
• cell pigment location-colour
  absent
and    membranaceously brownish
• cell contents presence-kind
  absent
• cell diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• cell length
  unknown
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  absent
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [with Hartig net like structures]
• hyphae arrangement
  without pattern
• hyphae vessel-like presence
  absent
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  unknown
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
16 µm    Maximum value
• cell length
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
37 µm    Maximum value
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle [but mantle lacking, apart from some scattered hyphae running towards the tip]
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
0 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0 µm    Mean (= average)
• calyptra cell remnants presence
  present
• mantle different layers presence
  not discernable
• unlayered mantle organization
  plectenchymatous
• unlayered mantle hyphae tangentially length
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
44 µm    Upper value of unspecified range (could be µ+s.d., but not known)
58 µm    Maximum value
• unlayered mantle hyphae radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
• shape
  rectangular [polyognal or flat]
• tangentially length
22 µm    Minimum value
26 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
6 µm    Minimum value
9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28 µm    Upper value of unspecified range (could be µ+s.d., but not known)
31 µm    Maximum value
• mean tangenial length TCt
1.5 µm    Minimum value
3.5 µm    Mean (= average)
8.2 µm    Maximum value

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular [to isodiametrical]
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
39 µm    Minimum value
44 µm    Lower value of unspecified range (could be µ-s.d., but not known)
83 µm    Upper value of unspecified range (could be µ+s.d., but not known)
89 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
22 µm    Minimum value
26 µm    Lower value of unspecified range (could be µ-s.d., but not known)
48 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
  unknown
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.1     Minimum value
1.9     Mean (= average)
3.3     Maximum value

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis [showing occasional gaps]
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  unknown
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  unknown
• structure {in plan view}
  infrequently lobed
• lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section
• calyptra cell remnants presence
  present
• calyptra cell remnants kind
  close to root cells

Anatomy of the tannin cells in cross-section
• presence
  present
• tangentially length
11 µm    Minimum value
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
61 µm    Maximum value
• radially diameter
8 µm    Minimum value
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33 µm    Upper value of unspecified range (could be µ+s.d., but not known)
44 µm    Maximum value
• mean shape-ratio TCq
0.4     Minimum value
1.6     Mean (= average)
3.3     Maximum value

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
28 µm    Minimum value
33 µm    Lower value of unspecified range (could be µ-s.d., but not known)
72 µm    Upper value of unspecified range (could be µ+s.d., but not known)
83 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
28 µm    Lower value of unspecified range (could be µ-s.d., but not known)
61 µm    Upper value of unspecified range (could be µ+s.d., but not known)
67 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.5     Minimum value
1.4     Mean (= average)
2.5     Maximum value

Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• intrahyphal hyphae clamps presence
  absent
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  absent
• anastomoses type
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  tortuous
or    irregularly inflated or even beaded
• cell pigment location-colour
  unknown
• pigment presence-distribution
  unknown
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
or    Y-shaped
• distance {from septum}
  adjacent to septum
• side-branches at septum number
  one side-branch at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  as thick as main part of branch

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
or    inflated, ampullate, trumpet-like at both sides
or    inflated at middle parts
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
or    ramified
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
6 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33 µm    Upper value of unspecified range (could be µ+s.d., but not known)
48 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  with soil particles
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  not applicable
• anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  uneven in thickness


Anatomy of the warts occouring on emanating hyphae
• presence
  absent
Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  consistently present
• shape
  as normal hyphae [repeatedly ramified]
• diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
16 µm    Maximum value

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [mantle and Hartig net hyphae dull yellowish brown, thin substrate hyphae brighter, greyish white or yellowish white, walls of tannin cells bright blue]
• mantle in section blue-filter, 450-490 nm presence
  absent
• mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  dextrinoid
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent
• reaction with sulpho-vanillin presence
  absent
• reaction with toluidin blue presence
  absent [? Blue staining hyphae belong possibly to a foreign fungus]

Ecology

• geographic occurrence continent
  North America
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant habitat kind
  forests, woods

Fungus

• family
  Balsamiaceae
• fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
4     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
360     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
350     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
180     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 4. band from below number
115     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
780     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
310     Mean (= average)

Remarks

• public notes
  EcoRI: 800 bp, 320 bp; TaqI: 550 bp, 310 bp, 200 bp

– Item listing –