Sistotrema aff. muscicola (Pers.) S. Lundell + Castanea

– Item listing –

Literature

• references
  Di Marino E, Scattolin L, Bodensteiner P, Agerer R (2008) Sistotrema is a genus with ectomycorrhizal species - confirmation of what sequence studies already suggested. Mycol Progress 7: 169-176.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pyramidal
• tips {per 10 mm} number
8     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
• ramification orders
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• main axis diameter
0.3 mm    Minimum value
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Maximum value
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
• exploration type
  medium distance fringe
Morphology of the unramified ends
• mantle hydrophobicity presence
  present
• shape
  bent
or    sinuous
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0.4 mm    Minimum value
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  ochre, yellowish brown [whitish ochre]
or    whitish
• older parts colour
  greyish
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    not smooth
• mantle surface {in detail} kind
  densely woolly
or    loosely woolly
• emanating hyphae presence
  present
Morphology of rhizomorphs
• diameter
0.02 mm    Minimum value
0.045 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.075 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.12 mm    Maximum value
• colour
  whitish
• connection to mantle kind
  oblique
• connection to substrate
  growing into the soil or organic layers
• margin habit
  woolly
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    emanating hyphae
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  ring-like arrangement of hyphal bundles (type A)
and    hyphae rather irregularly arranged and no special pattern discernible (type B)
and    gelatinous matrix between the hyphae (type C)
• clavate end cell cover presence
  absent
• matrix kind
  gelatinous
• hyphal system kind
  undifferentiated
• hyphae short protrusions presence
  absent
• hyphae hyphal junctions angle {between}
  ca. 45° and less
and    ca. 90°
• septa clamps presence
  present
• septa clamps abundance
  infrequent
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  membranaceously yellowish
• cell contents presence-kind
  oily droplets, which do not stain in sulpho-vanillin
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell wall thickness
0.1 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  plectenchymatous, ring-like [slightly]
• incomplete septa presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell contents presence-kind
  oily droplets, which do not stain in sulpho-vanillin
• cell wall thickness
0.1 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  ring-like [occasionally]
• hyphae vessel-like presence
  absent
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell contents presence-kind
  with oily droplets, which do not stain in sulpho-vanillin [some]
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
12.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  not discernable
• unlayered mantle organization
  plectenchymatous
• unlayered mantle hyphae tangentially length
1.2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• unlayered mantle hyphae radially diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
8.6 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.25     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  paraepidermal
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
1.2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  absent
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps surface kind
  smooth
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
or    inflated, ampullate, trumpet-like at one side
or    inflated at middle parts
• anatomy emanating elements emanating hyphae cell diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Mean (= average)


Anatomy of rhizomorphs
• type
  slightly differentiated; central hyphae somewhat enlarged
(type C)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  present, not specified
• contents presence-kind
  absent
• ampullate, trumpet-like inflated presence
  present
• ampullate inflations thickness
6 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• roundish plasmatically yellow cells presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  present, not specified
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  present, not specified
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  present, not specified
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  present, not specified
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• mantle in section UV-filter 340-380 nm presence
  absent
• mantle in section blue-filter, 450-490 nm presence
  absent
• mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

• reaction with cotton-blue-lactic-acid presence
  present [slightly bluish]
• reaction with FEA presence
  present [oily droplets not visible]
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Fagaceae
• plant genus
  Castanea
• plant habitat kind
  forests, woods

Fungus

• family
  Hydnaceae
• fruitbodies growth habit
  epigeous
or    resupinate-porioid

Remarks

• public notes
  Applying the currently used species concept, that is based on fruitbody anatomy, it can only be determined as S. muscicola, despite slight variations of morphological and anatomical features. The moderate identity values of ITS sequences obtained from fruitbody maerial of the here characterized Sistotrema specimen and the previously published S. muscicola collection are, however, clearly out of the range accepted for intraspecific sequence variability.

– Item listing –