Lactarius vellereus (Fr.) Fr. + Fagus

– Item listing –

Literature

• references
  Brand F (1991) Ektomykorrhizen an Fagus sylvatica. Charakterisierung und Identifizierung, ökologische Kennzeichnung und unsterile Kultivierung. Libri Botanici 2: 1-229.
Brand F, Agerer R (1986) Studien an Ektomykorrhizen VIII. Die Mykorrhizen von Lactarius subdulcis, Lactarius vellereus und Laccaria amethystina an Buche. Z Mykol 52(2): 287-320.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
40 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• tips {per 10 mm} number
12     Minimum value
38     Lower value of unspecified range (could be µ-s.d., but not known)
42     Upper value of unspecified range (could be µ+s.d., but not known)
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
Morphology of the unramified ends
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
• diameter
0.25 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.35 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
• very tip colour
  whitish
• older parts colour
  brown
or    red
or    grey
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle laticifers visibility
  present
• mantle secreted latex {after scratching mantle} presence
  present
• mantle secreted latex {after scratching mantle} colour-habit
  white, remaining white after some minutes
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  shiny
or    smooth
• emanating hyphae presence
  absent
or    present
• emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
• cross-section shape
  round or roundish
• colour
  whitish
• ramification kind-frequency
  infrequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  not specific
or    proximal
• margin habit
  smooth
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
or    present
• location
  middle mantle layer
or    inner mantle layer
• shape
  straight and even
• ramification presence-abundance
  infrequent
• cell diameter
3 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
• mantle type
  epidermoid cells (type M)
• matrix kind
  gelatinous
• pores between cells presence
  present
• pores between cells abundance
  infrequent
• septa thickness {relative to cell walls}
  thinner than walls
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell density
5.7     Mean (= average)
• cell wall thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• cell wall surface habit
  smooth
• cell wall projections presence
  present
• cell wall projections abundance
  abundant
• cell wall projections shape
  even in thickness
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous
• matrix kind
  not gelatinous
• cell pigment location-colour
  membranaceously brownish
• cell contents presence-kind
  absent
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
or    gelatinous
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
35 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  absent


Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  plectenchymatous
or    pseudoparenchymatous
• middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  absent


Anatomy of the Hartig net in cross-section
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
or    beaded
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
• lobes width
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• structure {in plan view}
  infrequently lobed
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• backwards-oriented clamps presence
  absent
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
• distance {from septum}
  adjacent to septum
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  absent
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs
• type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• nodia presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
2 µm    Mean (= average)
• length
2 µm    Mean (= average)
• distance {between each other}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with ruthenium-red presence
  present
• reaction with sudan III presence
  present
• reaction with sulpho-vanillin presence
  present
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Fagaceae
• plant genus
  Fagus
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Lactarius sect. Lactarius
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mycorrhizal systems mostly pyramidal; mycorrhizal ends slightly brownish to nut-brown, older parts flesh-coloured to red-brwon then darker grey-brown to grey; middle mantle layers in plan view with slightly membranaceously brownish cells; septa of laticifers infrequent; hyphae of very tip (outer mantle layers) with 1-2 um thick lumen, walls 2.5-4 um thick; rhizomorph hyphae with 1-2 um thick walls close to mantle; in cross-section Hartig net 1-2(-3) cortical cell layers deep, in older parts protruding towards endodermis; autofluorescence of mantle in section with UV-filter outer layers distisnctly light blue and middle mantle layers olive-yellow and inner mantle layers slightly blue, with blue-filter greenish yellow and middle mantle layers rather greenith orange; mantle in sulfo-vanillin partly slightly violet-brown, latex without reaction, in KOH latex grey, in brillant-cresyl-blue violet-blue, in toluidin-blue blue-violet, in cotton-blue walls of outer mantle layers distinctly blue, in sudan III latex distinctly red-orange, in ruthenium-red walls of outer mantle layer red, in chlorazol black slightly blue-green, in phloxin B brown-red; (after Brand (1991) possibly misidentified, this mycorrhiza was also found associated with L. blennius!).

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