Amphinema byssoides (Pers. : Fr.) J. Erikss. + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R (1987-1995) Colour Atlas of Ectomycorrhizae. 1st-9th delivery. Einhorn, Schwäbisch Gmünd.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Danielson RM, Pruden M (1989) The mycorrhizal status of urban spruce. Mycologia 81(3): 335-341.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest, a microscopical atlas. Kernforschungszentrum, Karlsruhe.
Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.
Weiss M (1988) Ektomykorrhizen von Picea abies. Synthese, Ontogenie und Reaktion auf Umweltschadstoffe. Diss Univ München.
Weiss M (1989) Amphinema byssoides. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 23, Einhorn-Verlag, Schwäbisch Gmünd.
Weiss M (1990) Studien an Ektomykorrhizen XXV. Untersuchungen zur Ontogenie von Ektomykorrhizen an Picea abies. Nova Hedwigia 50: 361-393.
Weiss M, Agerer R (1988) Studien an Ektomykorrhizen XII. Drei nichtidentifizierte Mykorrhizen an Picea abies (L.) Karst. aus einer Baumschule. Eur. J. For. Path. 18: 26-43.

Morphology

Morphology of the mycorrhizal system
• length
4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
• exploration type
  medium distance fringe
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
• diameter
0.2 mm    Minimum value
0.25 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
• colour
  yellow
• very tip colour
  whitish
• older parts colour
  brown
or    yellow
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  absent
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
• mantle surface {in detail} kind
  densely woolly
or    densely cottony
or    densely stringy
or    loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
• emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
• diameter
0.008 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  yellow
• ramification kind-frequency
  repeatedly into smaller filaments
• connection to mantle kind
  fan-like
• origin location
  not specific
or    proximal
• margin habit
  densely enveloped by hyphae
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, not constricted at septa
• cell contents presence-kind
  absent
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  rough
and    with few crystals
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
4 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
24 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mantle different layers presence
  not discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
40 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• mean tangenial length TCt
78.8 µm    Mean (= average)
• mean shape-ratio TCq
9.3     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
62.7 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
4.9     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
• mantle different layers presence
  not discernible
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• mean tangential length TCt
39.3 µm    Mean (= average)
• mean shape-ratio TCq
4.6     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
23.9 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.9     Mean (= average)

Anatomy of the Hartig net in cross-section
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• anastomoses type
  closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
• anastomoses surface habit
  rough or with crystals
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  wavy
• cell pigment location-colour
  absent
or    membranaceously yellowish
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
or    Y-shaped
• distance {from septum}
  one or two hyphal diam. below the septum
or    in considerable distance from the septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps width {relative to hypha in dorsal view}
  as broad as
• clamps outline {in lateral view}
  as a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
or    present
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  absent
or    present
• abundance
  infrequent
• distribution
  not specified

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
15 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
150 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    with crystals
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  elongated, perpendicular warts
• anatomy emanating elements emanating hyphae cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• contents presence-kind
  absent
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• {in foreign ectomycorrhizae} presence
  absent
• shape
  globular
• diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  oval
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• distance {between each other}
15 µm    Minimum value
22 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome
• hyphae warts shape
  acicular, with a translucent channel
• mantle matrix presence-kind
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  absent
or    present
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
or    present
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
or    present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity haustoria presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent
• reaction with sulpho-vanillin presence
  absent
or    present
• reaction with toluidin blue presence
  present

Ecology

• substrate
  in organic layer
• geographic occurrence continent
  Europe
or    North America
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  nursery
or    forests, woods

Fungus

• family
  Atheliaceae ("Corticiaceae")
• fruitbodies growth habit
  epigeous
or    corticioid

Remarks

• public notes
  Mycorrhizal systems irregularly monopodial-pinnate; mycorrhizal ends sometimes slightly bent, yellow or yellowish white; autofluorescence of mantle in section with UV-filter yellowish-green or bluish white, with blue-filter yellow, with green-filter red; mantle in sulfo-vanillin with slightly reddish then dark grey cell walls, in KOH turning bright yellow, in FeSO4 cytoplasm slightly grey, in brillant-cresyl-blue cell walls and cytoplasm bluish, in toluidin-blue cell walls violet-blue, in acid fuchsin cell walls red.

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