Amphinema byssoides (Pers. : Fr.) J. Erikss. + Picea

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– Enlarged view –

Literature

references
  Agerer R (1987-1995) Colour Atlas of Ectomycorrhizae. 1st-9th delivery. Einhorn, Schwäbisch Gmünd.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Danielson RM, Pruden M (1989) The mycorrhizal status of urban spruce. Mycologia 81(3): 335-341.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest, a microscopical atlas. Kernforschungszentrum, Karlsruhe.
Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.
Weiss M (1988) Ektomykorrhizen von Picea abies. Synthese, Ontogenie und Reaktion auf Umweltschadstoffe. Diss Univ München.
Weiss M (1989) Amphinema byssoides. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 23, Einhorn-Verlag, Schwäbisch Gmünd.
Weiss M (1990) Studien an Ektomykorrhizen XXV. Untersuchungen zur Ontogenie von Ektomykorrhizen an Picea abies. Nova Hedwigia 50: 361-393.
Weiss M, Agerer R (1988) Studien an Ektomykorrhizen XII. Drei nichtidentifizierte Mykorrhizen an Picea abies (L.) Karst. aus einer Baumschule. Eur. J. For. Path. 18: 26-43.

Morphology

Morphology of the mycorrhizal system
length
4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  solitary or in small numbers
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
exploration type
  medium distance fringe
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
diameter
0.2 mm    Minimum value
0.25 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
colour
  yellow
very tip colour
  whitish
older parts colour
  brown
or    yellow
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  absent
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
mantle surface {in detail} kind
  densely woolly
or    densely cottony
or    densely stringy
or    loosely stringy
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
diameter
0.008 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  yellow
ramification kind-frequency
  repeatedly into smaller filaments
connection to mantle kind
  fan-like
origin location
  not specific
or    proximal
margin habit
  densely enveloped by hyphae
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal system kind
  undifferentiated
septa thickness {relative to cell walls}
  as thick as walls
cell shape
  cylindric, not constricted at septa
cell contents presence-kind
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  rough
and    with few crystals
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
hyphae arrangement
  plectenchymatous, without pattern
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
4 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
24 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
mantle different layers presence
  not discernable
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
40 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
mean tangenial length TCt
78.8 µm    Mean (= average)
mean shape-ratio TCq
9.3     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
62.7 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
4.9     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
mantle different layers presence
  not discernible
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  present
rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
shape
  tangentially-oval to tangentially-elliptic
tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
mean tangential length TCt
39.3 µm    Mean (= average)
mean shape-ratio TCq
4.6     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
23.9 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.9     Mean (= average)

Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around tannin cells number
  one
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
anastomoses type
  closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
anastomoses surface habit
  rough or with crystals
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  wavy
cell pigment location-colour
  absent
or    membranaceously yellowish
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  approximately 90°
or    Y-shaped
distance {from septum}
  one or two hyphal diam. below the septum
or    in considerable distance from the septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps width {relative to hypha in dorsal view}
  as broad as
clamps outline {in lateral view}
  as a semicircle
or    not constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent
or    present
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  absent
or    present
abundance
  infrequent
distribution
  not specified

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
15 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
150 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    with crystals
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall surface structures shape
  elongated, perpendicular warts
anatomy emanating elements emanating hyphae cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
nodia presence
  absent
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
{in foreign ectomycorrhizae} presence
  absent
shape
  globular
diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  oval
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
distance {between each other}
15 µm    Minimum value
22 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
Ultrastructure
septal pores type
  as dolipores with perforated parenthesome
hyphae warts shape
  acicular, with a translucent channel
mantle matrix presence-kind
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  absent
or    present
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  absent
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
or    present
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
or    present
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
amyloidity haustoria presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with ruthenium-red presence
  absent
reaction with sulpho-vanillin presence
  absent
or    present
reaction with toluidin blue presence
  present

Ecology

substrate
  in organic layer
geographic occurrence continent
  Europe
or    North America
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  nursery
or    forests, woods

Fungus

family
  Atheliaceae ("Corticiaceae")
fruitbodies growth habit
  epigeous
or    corticioid

Remarks

public notes
  Mycorrhizal systems irregularly monopodial-pinnate; mycorrhizal ends sometimes slightly bent, yellow or yellowish white; autofluorescence of mantle in section with UV-filter yellowish-green or bluish white, with blue-filter yellow, with green-filter red; mantle in sulfo-vanillin with slightly reddish then dark grey cell walls, in KOH turning bright yellow, in FeSO4 cytoplasm slightly grey, in brillant-cresyl-blue cell walls and cytoplasm bluish, in toluidin-blue cell walls violet-blue, in acid fuchsin cell walls red.

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