Ramaria subbotrytis (Coker) Corner + Quercus

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R (1996) Ramaria subbotrytis (Coker) Corner + Quercus robur L. Descr Ectomyc 1: 125-130.
Agerer R (1998) Ramaria subbotrytis. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 134, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  nest-like, hyphal mats
• main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.33 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  present
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
• exploration type
  medium distance mat
Morphology of the unramified ends
• shape
  straight
or    bent
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
3 mm    Maximum value
• diameter
0.24 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  white
• very tip colour
  white
• older parts colour
  violet, dark reddish blue
or    white
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
or    not smooth
• mantle surface {in detail} kind
  loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 mm    Maximum value
• cross-section shape
  round or roundish
or    flat
• colour
  white
• ramification kind-frequency
  repeatedly into smaller filaments
• connection to mantle kind
  oblique
• origin location
  not specific
or    proximal
• margin habit
  hairy
or    fan-like
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
and    gelatinous matrix between the hyphae (type C)
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  thicker than walls
• septa clamps presence
  absent
• cell shape
  cylindric, constricted at septa
and    ampullate at one side of septum
• cell pigment location-colour
  absent
• cell contents presence-kind
  {in some cells} homogeneous, brownish
• cell diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• cell length
5 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
135 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
0.5 µm    Maximum value
• cell wall surface habit
  smooth
and    with few soil particles
and    with many soil particles
• drops of exuded pigment presence
  present
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  plectenchymatous, ring-like
• cell pigment location-colour
  colourless
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• cell length
8 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
140 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• cell pigment location-colour
  absent
• cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4.5 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
15 µm    Minimum value
20 µm    Mean (= average)
30 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
32 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
63 µm    Upper value of unspecified range (could be µ+s.d., but not known)
68 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
15.2 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.3     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  periepidermal
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
or    beaded
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  infrequently lobed
• lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  absent

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
12 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm    Minimum value
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
18.5 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1     Mean (= average)

Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• septal pores configuration
  globular thickenings
• backwards-oriented ramifications presence
  present
• backwards-oriented clamps presence
  absent
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  as acanthocystidia (type P)
• septa presence
  absent
• septa kind
  absent, with basal clamp only
• diameter {proximal}
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  similar
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall thickness {relative to mantle cells}
  similar in thickness
• cell wall evenness
  even in thickness
• surface habit
  smooth
• contents presence
  present
• contents type
  finely granular

Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  absent
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
1.5 µm    Minimum value
2 µm    Mean (= average)
2.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
15 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
or    as thick as
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  slightly differentiated; central hyphae somewhat enlarged
(type C)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  filled with brownish or yellowish substances
• ampullate, trumpet-like inflated presence
  present
• ampullate inflations thickness
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
• anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
1 µm    Mean (= average)
• length
1 µm    Mean (= average)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with anilin presence
  absent
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  present
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  present
• reaction with phenole-aniline presence
  absent
• reaction with sulpho-vanillin presence
  absent

Ecology

• substrate
  in mineral soil
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Fagaceae
• plant genus
  Quercus
• plant habitat kind
  forests, woods

Fungus

• family
  Ramariaceae
• fruitbodies growth habit
  epigeous
or    clavarioid

Remarks

• public notes
  Mycorrhizal systems irregular monopodial-pinnate to monopodial-pyramidal; mycorrhizal ends only slightly tortuous, if air between mantle hyphae displaced by water slightly brownish, older parts with a slightly violet tint; mantle in plan view with infrequent at one side ampullate hyphae and there 5.5-7.5 um thick, drops (?, possibly tenderly walled cells) of exuded pigment 5-13x5-8 um; cystidia infrequent on mantle, more abundant on rhizomorphs, outgrowths of cystidia 2-6 um long, (0.5-)1(-1.5) um thick; in cross-section Hartig net (1-)2(-3) cortical cell layers deep; autofluorescence of mantle in section with UV-filter blue, with blue-filter green, with green-filter red; mantle in guaiac blue and more distinctly in rhizomorphs, in cotton-blue weakly bluish, in phenole walls slightly greyish, in safranine walls red.

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