Tuber brumale Vitt. + Quercus

– Item listing –

Literature

• references
  Zambonelli A, Salomoni S, Pisi A (1993) Caratterizzazione anatomo-morfologica delle micorrize di Tuber spp. su Quercus pubescens Willd. Micol Ital (3): 73-90.
Fischer CR, Suz LM, Martin MP, Colinas C (2004) Tuber brumale Vitt. + Quercus ilex L. Descr Ectomyc 7/8: 135-141.

Morphology

Morphology of the mycorrhizal system
• length
1.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate
or    monopodial-pyramidal
• main axis diameter
0.24 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
or    beaded [occasionally]
• shape {of distal end}
  not inflated, cylindric
or    inflated, club-shaped
• length
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 mm    Maximum value
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  dark brown
or    brown
or    ochre, yellowish brown
• very tip colour
  ochre, yellowish brown
• older parts colour
  dark brown [to brown-grey]
or    brown
• mantle {distinct} surface visibility
  present
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  densely short-spiny
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  concentrated distally
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  epidermoid cells (type M)
• hyphae arrangement
  not specific
• pores between cells presence
  absent
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
and    membranaceously brownish
• cell density
4     Minimum value
4.2     Lower value of unspecified range (could be µ-s.d., but not known)
6.4     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
• cell wall projections presence
  absent
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell shape
  polygonal [irregularly polygonal]
• cell pigment location-colour
  membranaceously brownish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• cell length
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
• cell density
10     Lower value of unspecified range (could be µ-s.d., but not known)
12     Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  pseudoparenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
23 µm    Maximum value
• cell density
7     Lower value of unspecified range (could be µ-s.d., but not known)
12     Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
4     Minimum value
5     Lower value of unspecified range (could be µ-s.d., but not known)
7     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
16 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
78 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• calyptra cell remnants presence
  present
• calyptra cell remnants type
  in one row
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• outer mantle layer hyphae radially diameter
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
5.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
3.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
3.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
4.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)



Anatomy of the emanating elements in general
• intrahyphal hyphae clamps presence
  absent
• backwards-oriented clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  awl-shaped, bristle-like (type A)
• ramification presence-position
  absent
• septa kind
  simple
• septa number
1     Mean (= average)
• basal clamp walls abruptly thick
  absent
• foot cell presence
  present
• diameter {proximal}
3.2 µm    Minimum value
3.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• diameter {distal}
0.8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
21 µm    Minimum value
58 µm    Lower value of unspecified range (could be µ-s.d., but not known)
135 µm    Upper value of unspecified range (could be µ+s.d., but not known)
155 µm    Maximum value
• cell wall colour
  yellowish
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• surface habit
  smooth
• contents presence
  absent

Anatomy of emanating hyphae
• cell pigment location-colour
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value


Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  absent
• mantle in section blue-filter, 450-490 nm presence
  present [slightly yellow]
• mantle in section green-filter, 530-560 nm presence
  present [slightly red]

Chemical reactions

• reaction with acetic acid presence
  absent
• reaction with acid fuchsin presence
  unknown
• reaction with AgNO3 presence
  unknown
• reaction with anilin presence
  present [walls swelling up]
• reaction with brillant-cresyl-blue presence
  present [cell walls dark blue, cystidia walls slightly blue]
• reaction with cotton-blue-lactic-acid presence
  present [mantle cell walls blue, cystidia unstained]
• reaction with ethanol 70% presence
  absent
• reaction with FEA presence
  unknown
• reaction with FeSO4 presence
  present [better contrast of cystidia which take a pale grey colour]
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with HCl presence
  unknown
• reaction with KJ presence
  unknown
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  unknown
• reaction with Melzer's reagent presence
  dextrinoid
• amyloidity or dextrinoidity location
  mantle hyphae
• reaction with methylene blue presence
  unknown
• reaction with phenole presence
  unknown
• reaction with phenole-aniline presence
  unknown
• reaction with ruthenium-red presence
  unknown
• reaction with sudan III presence
  unknown
• reaction with sudan IV presence
  unknown
• reaction with sulpho-vanillin presence
  absent
• reaction with sulphuric acid presence
  unknown
• reaction with toluidin blue presence
  present [manlte dark blue, cystidial walls uncoloured]
• reaction with trypan blue presence
  unknown
• reaction with water presence
  unknown

Ecology

• soil pH
  pH more than 7
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Fagaceae
• plant genus
  Quercus
• plant habitat kind
  nursery

Fungus

• family
  Tuberaceae
• fruitbodies growth habit
  hypogeous

Remarks

• public notes
  Mycorrhizal ends ochre-brown; cells of inner mantle layers in plan view epidermoid; in longitudinal section one cortical cell layer with Hartig net, in cross-section 1-2 cortical cell layers with Hartig net (Zambonelli et al. 1993).

– Item listing –