Russula atroglauca Einhellinger + Betula

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Literature

references
  Beenken L (2001) Russula atroglauca Einhellinger + Betula pubescens Ehrh. Descr Ectomyc 5: 125-130.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  abundant, dense
main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  short distance
Morphology of the unramified ends
mantle hydrophobicity presence
  present [at patches]
shape
  straight
or    bent [slightly]
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
3 mm    Maximum value
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brownish
or    ochre, yellowish brown
very tip colour
  ochre, yellowish brown [light]
after injury colour
  unchanged
older parts colour
  unknown
mantle cortical cells visibility
  unknown
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  densely short-spiny
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
or    cystidia
emanating elements cystidia location
  on outer mantle layer
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
matrix kind
  gelatinous
hyphae short protrusions presence
  present
hyphae sticky matrix on hyphal surface presence
  unknown
hyphae hyphal junctions angle {between}
  ca. 90°
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell shape
  cylindric, not constricted at septa
and    cylindric, constricted at septa
cell pigment location-colour
  absent
and    membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
2 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
  unknown
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous
matrix kind
  gelatinous
hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, ring-like
cell pigment location-colour
  unknown
cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely]
matrix kind
  unknown
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  unknown
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
25 µm    Mean (= average)
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  present
calyptra cell remnants type
  unknown
mantle different layers presence
  not discernable
unlayered mantle organization
  plectenchymatous
unlayered mantle hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
unlayered mantle hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  paraepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish [rectangular]
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type [irregularlyl lobed]
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  unknown
intrahyphal hyphae clamps presence
  absent
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  flask-shaped with an apical knob (type D)
apical knobs number
1     Mean (= average)
apical knobs arrangement
  symmetrically
apical knobs shape
  globose
apical knobs diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs wall thickness
0.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs cell wall thickness {relative to cystidial body}
  thicker than those of cystidial body
septa presence
  absent
foot cell presence
  absent
diameter {proximal}
6 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
  unknown
length
11 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  yellowish
cell wall colour {relative to mantle cells}
  unknown
cell wall thickness
0.5 µm    Minimum value
0.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  thicker
cell wall evenness
  unknown
surface habit
  smooth
contents presence
  absent
or    present
contents type
  brownish, with latex-like droplets

Anatomy of cystidia (type 2)
type
  awl-shaped, bristle-like (type A)
or    bottle-shaped with a straight neck (type B)
ramification presence-position
  absent
septa presence
  present
septa kind
  simple
septa distance
  unknown
septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
6     Maximum value
basal clamp walls abruptly thick
  absent
foot cell presence
  absent
diameter {proximal}
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  yellowish
cell wall colour {relative to mantle cells}
  darker
cell wall thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall evenness
  uneven in thickness
cell wall thickness {relative to mantle cells}
  thicker
surface habit
  smooth
contents presence
  absent
Anatomy of emanating hyphae
cell pigment location-colour
  absent
or    membranaceously yellowish
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Mean (= average)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [yellow-green throughout]
mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
mantle in section green-filter, 530-560 nm presence
  present [red throughout]

Chemical reactions

reaction with acid fuchsin presence
  present [walls slightly pink]
reaction with cotton-blue-lactic-acid presence
  present [walls blue]
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [walls pink, contents of many knob-bearing cystidia becoming dark brown]

Ecology

substrate
  in peat
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Betulaceae
plant genus
  Betula
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
465     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
180     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
10     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
320     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
255     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
50     Mean (= average)

Remarks

public notes
  EcoRI: 340 bp, 310 bp; TaqI: 300 bp, 90 bp, 75 bp, 30 bp

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