Inocybe obscurobadia (J. Favre) Grund & D. E. Stuntz + Picea

Literature

• references
	Beenken L (1996) Inocybe obscurobadia. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 96, Einhorn-Verlag, Schwäbisch Gmünd Beenken L, Agerer R, Bahnweg G (1996) Inocybe obscurobadia (J. Favre) Grund & D. E. Stuntz + Picea abies (L.) Karst. Descr Ectomyc 1: 47-52.

Morphology

Morphology of the mycorrhizal system

• length
0 mm	Lower value of unspecified range (could be µ-s.d., but not known)
10 mm	Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
	monopodial-pinnate
or	monopodial-pyramidal
• ramification orders
0	Lower value of unspecified range (could be µ-s.d., but not known)
2	Upper value of unspecified range (could be µ+s.d., but not known)
• main axis diameter
0.7 mm	Mean (= average)
• rhizomorphs as stout, short, conical structures presence-abundance
	absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
	absent
• rhizomorphs presence
	absent
• exploration type
	short distance

Morphology of the unramified ends

• shape
	straight
or	bent
or	beaded
• shape {of distal end}
	not inflated, cylindric
• length
0 mm	Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm	Upper value of unspecified range (could be µ+s.d., but not known)
3 mm	Maximum value
• diameter
0.25 mm	Lower value of unspecified range (could be µ-s.d., but not known)
0.35 mm	Upper value of unspecified range (could be µ+s.d., but not known)
• colour
	brown
• very tip colour
	white
• older parts colour
	dark brown
• mantle cortical cells visibility
	not visible
• mantle {distinct} surface visibility
	present
• mantle laticifers visibility
	absent
• mantle dots presence-colour
	absent
• mantle carbonizing presence
	absent
• mantle surface {in general} habit
	silvery
or	smooth
or	not smooth
• mantle surface {in detail} kind
	loosely cottony

Morphology of sclerotia

• presence
	absent

Anatomy

Anatomical features of the entire mycorrhiza

Anatomy of laticifers

• presence
	absent

Anatomical features of the mantle

Anatomy of the outer mantle layer apart from the ectormycorrhizal tip

• organisation
	plectenchymatous
• mantle type
	ring-like arrangement of hyphal bundles (type A)
and	hyphae rather irregularly arranged and no special pattern discernible (type B)
and	hyphae arranged net-like, repeatedly and squarrosely branched (type E)
• hyphal system kind
	undifferentiated
• septa thickness {relative to cell walls}
	as thick as walls
• septa clamps presence
	absent
• cell shape
	cylindric, not constricted at septa
• cell pigment location-colour
	absent
• cell contents presence-kind
	absent
• cell diameter
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
5 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
15 µm	Lower value of unspecified range (could be µ-s.d., but not known)
60 µm	Upper value of unspecified range (could be µ+s.d., but not known)
80 µm	Maximum value
• cell wall thickness
0.2 µm	Mean (= average)
• cell wall surface habit
	smooth
• drops of exuded pigment presence
	absent

Anatomy of the middle mantle layer

• organisation
	plectenchymatous
or	pseudoparenchymatous
• hyphae arrangement
	plectenchymatous, without pattern
• cell pigment location-colour
	colourless
• cell diameter
3 µm	Lower value of unspecified range (could be µ-s.d., but not known)
10 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
10 µm	Lower value of unspecified range (could be µ-s.d., but not known)
30 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
	absent
• cell wall thickness
0.2 µm	Mean (= average)
• cell wall surface habit
	smooth

Anatomy of the inner mantle layer

• organisation
	plectenchymatous
• hyphae arrangement
	with broad streaks of parallel hyphae
• septa clamps presence
	absent
• cell diameter
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
5 µm	Upper value of unspecified range (could be µ+s.d., but not known)
8 µm	Maximum value
• cell length
5 µm	Lower value of unspecified range (could be µ-s.d., but not known)
40 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
	with oily droplets, which do not stain in sulpho-vanillin

Anatomy of the outer mantle layer of the ectormycorrhizal tip

• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
	like other parts of mantle

Anatomy of the mantle in longitudinal section

• mantle thickness {apart from tip}
15 µm	Minimum value
20 µm	Lower value of unspecified range (could be µ-s.d., but not known)
25 µm	Upper value of unspecified range (could be µ+s.d., but not known)
30 µm	Maximum value
• mantle thickness {at ectomycorrhizal tip}
15 µm	Lower value of unspecified range (could be µ-s.d., but not known)
20 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
	discernable
• outer mantle layer organisation
	plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
5 µm	Upper value of unspecified range (could be µ+s.d., but not known)
20 µm	Maximum value
• outer mantle layer hyphae radially diameter
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
3 µm	Upper value of unspecified range (could be µ+s.d., but not known)
5 µm	Maximum value
• inner mantle layer organisation
	plectenchymatous
• inner mantle layer hyphae tangentially length
1.5 µm	Lower value of unspecified range (could be µ-s.d., but not known)
3 µm	Upper value of unspecified range (could be µ+s.d., but not known)
7 µm	Maximum value
• inner mantle layer hyphae radially diameter
1.5 µm	Lower value of unspecified range (could be µ-s.d., but not known)
2 µm	Upper value of unspecified range (could be µ+s.d., but not known)
5 µm	Maximum value

Anatomy of the tannin cells in longitudinal section

• presence
	present
• rows number
1	Mean (= average)
• shape
	tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
15 µm	Lower value of unspecified range (could be µ-s.d., but not known)
80 µm	Upper value of unspecified range (could be µ+s.d., but not known)
90 µm	Maximum value
• radially diameter
5 µm	Lower value of unspecified range (could be µ-s.d., but not known)
15 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
48.5 µm	Mean (= average)
• mean shape-ratio TCq
5.8	Mean (= average)

Anatomy of the cortical cells in longitudinal section

• anatomy mantle longitudinal section cortical (epidermal) cells shape
	tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
20 µm	Minimum value
30 µm	Lower value of unspecified range (could be µ-s.d., but not known)
100 µm	Upper value of unspecified range (could be µ+s.d., but not known)
120 µm	Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10 µm	Lower value of unspecified range (could be µ-s.d., but not known)
40 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
71.5 µm	Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.2	Mean (= average)

Anatomy of the Hartig net in longitudinal section

• presence
	present
• kind
	protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
	roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
3 µm	Upper value of unspecified range (could be µ+s.d., but not known)
5 µm	Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
	one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
	roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm	Lower value of unspecified range (could be µ-s.d., but not known)
3 µm	Upper value of unspecified range (could be µ+s.d., but not known)
5 µm	Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
	one
• structure {in plan view}
	of palmetti type
• lobes width
1 µm	Lower value of unspecified range (could be µ-s.d., but not known)
2 µm	Upper value of unspecified range (could be µ+s.d., but not known)
3 µm	Maximum value

Anatomy of the mantle in cross-section

• mantle different layers presence
	discernible
• outer mantle layer organisation
	plectenchymatous
• inner mantle layer organisation
	plectenchymatous

Anatomy of the tannin cells in cross-section

• presence
	present
• rows number
1	Mean (= average)
2	Maximum value
• shape
	tangentially-oval to tangentially-elliptic
• tangentially length
15 µm	Lower value of unspecified range (could be µ-s.d., but not known)
45 µm	Upper value of unspecified range (could be µ+s.d., but not known)
50 µm	Maximum value
• radially diameter
4 µm	Lower value of unspecified range (could be µ-s.d., but not known)
10 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangential length TCt
32.6 µm	Mean (= average)
• mean shape-ratio TCq
5.2	Mean (= average)

Anatomy of the cortical cells in cross-section

• anatomy mantle cross-section cortical (epidermal) cells shape
	tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm	Lower value of unspecified range (could be µ-s.d., but not known)
40 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm	Lower value of unspecified range (could be µ-s.d., but not known)
40 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
25.7 µm	Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.2	Mean (= average)

Anatomy of the emanating elements in general

• septal pores configuration
	globular thickenings
• backwards-oriented clamps presence
	absent
• anastomoses type
	open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
	as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
	as thick as

Anatomy of cystidia

Anatomy of emanating hyphae

• shape
	not striking
• cell pigment location-colour
	absent
• drops of exuded pigment presence
	absent

Anatomy of the ramification of emanating hyphae

• presence
	present
• kind
	approximately 90°
• distance {from septum}
	in considerable distance from the septum
• side-branches at septum number
	one side-branch at septum

Anatomy of the septa of emanating hyphae

• clamps presence
	present
• clamps outline {in lateral view}
	as a semicircle
or	constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
	as broad as
• clamps hole presence
	absent
• clamps blister-like structure {at basis} presence
	absent

Anatomy of secondary septa of emanating hyphae

• presence
	present
• abundance
	infrequent
• distribution
	not specified

Anatomy of the cells of emanating hyphae

• anatomy emanating elements emanating hyphae cell shape
	even
or	with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell shape {at distal end}
	simple
• anatomy emanating elements emanating hyphae cell diameter
1.5 µm	Lower value of unspecified range (could be µ-s.d., but not known)
2 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
	smooth
or	without lens-shaped appositions
or	without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm	Mean (= average)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
	as thick as
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
	even in thickness

Anatomy of rhizomorphs

• type
	lacking, only emanating hyphae present (type G)

Anatomy of hyphae in rhizomorphs

Anatomy of chlamydospores

• presence
	absent

Anatomy of haustoria

• {of ectomycorrhiza former} presence
	absent
• {of foreign origin} presence
	absent

Anatomy of nuclei

• number {per cell}
2	Mean (= average)
• shape
	round
• diameter
1 µm	Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• length
1 µm	Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm	Upper value of unspecified range (could be µ+s.d., but not known)
• distance {between each other}
0 µm	Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm	Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
	absent
• whole mycorrhizae UV 366 nm colour-presence
	yellow, yellowish
• mantle in section UV-filter 340-380 nm presence
	present
• mantle in section blue-filter, 450-490 nm presence
	present
• mantle in section green-filter, 530-560 nm presence
	present

Chemical reactions

• reaction with acid fuchsin presence
	present
• reaction with anilin presence
	absent
• reaction with brillant-cresyl-blue presence
	present
• reaction with cotton-blue-lactic-acid presence
	present
• reaction with ethanol 70% presence
	absent
• reaction with FeSO4 presence
	absent
• reaction with formol 40% presence
	absent
• reaction with guaiac presence
	absent
• reaction with KOH 10% presence
	absent
• reaction with lactic acid presence
	absent
• reaction with Melzer's reagent presence
	absent
• reaction with phenole-aniline presence
	absent
• reaction with ruthenium-red presence
	absent
• reaction with sulpho-vanillin presence
	present

Ecology

• geographic occurrence continent
	Europe
• knowledge about association with foreign fruitbodies presence
	unknown

Tree

• plant family
	Pinaceae
• plant genus
	Picea
• plant habitat kind
	gardens, parks

Fungus

• family
	Cortinariaceae
• fruitbodies growth habit
	epigeous
or	pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
1	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
660	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
4	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
28	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
60	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
140	Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 4. band from below number
255	Mean (= average)

Remarks

• public notes

Autofluorescence of mantle in section with UV-filter greenish blue throughout, with blue-filter yellow throughout, with green-filter red throughout; mantle in sulfo-vanillin with orange red cell contents, in KOH walls appearing more distinct, in brillant-cresyl-blue walls slightly blue, in cotton-blue walls dark blue and single septa of emanating hyphae dark blue, in anilin walls dissolving, in acid fuchsin walls slightly pink.

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