Rhizopogon subcaerulescens A. H. Smith + Tsuga

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– Enlarged view –

Literature

references
  Agerer R (1996) Rhizopogon subcaerulescens. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 104, Einhorn-Verlag, Schwäbisch Gmünd.
Agerer R, Müller WR, Bahnweg G (1996) Ectomycorrhizae of Rhizopogon subcaerulescens on Tsuga heterophylla. Nova Hedwigia 63.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
40 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
abundance
  abundant, dense
main axis diameter
0.6 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.75 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  long distance
Morphology of the unramified ends
shape
  straight
or    bent
or    sinuous
or    tortuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.9 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6.5 mm    Maximum value
diameter
0.36 mm    Minimum value
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.51 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  lilac, light reddish blue
or    white
very tip colour
  whitish
older parts colour
  brown
or    red
or    grey
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    smooth
or    not smooth
mantle surface {in detail} kind
  densely short-spiny
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.26 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  concolourous to mantle
or    brownish
or    lilac, light reddish blue
or    whitish
ramification kind-frequency
  infrequently, at restricted points
connection to mantle kind
  distinct
origin location
  proximal
margin habit
  smooth
or    hairy
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    cystidia
emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  ring-like arrangement of hyphal bundles (type A)
matrix kind
  gelatinous
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  absent
cell shape
  cylindric, not constricted at septa
and    irregularly inflated or even beaded
cell pigment location-colour
  plasmatically yellowish
and    epimembranaceously brownish
cell contents presence-kind
  absent
cell diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1.5 µm    Maximum value
cell wall surface habit
  with scar-like structures
and    with many crystals
crystals length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
drops of exuded pigment presence
  present
Anatomy of the middle mantle layer
organisation
  plectenchymatous
matrix kind
  not gelatinous
hyphae arrangement
  plectenchymatous, ring-like
cell pigment location-colour
  colourless
cell diameter
5 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Mean (= average)
1 µm    Maximum value
cell wall surface habit
  smooth
or    rough
crystals length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  star-like
or    ring-like
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
cell length
7.5 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
15 µm    Minimum value
20 µm    Mean (= average)
30 µm    Maximum value
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
inner mantle layer hyphae radially diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
95 µm    Upper value of unspecified range (could be µ+s.d., but not known)
105 µm    Maximum value
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
mean tangenial length TCt
74.4 µm    Mean (= average)
mean shape-ratio TCq
7.9     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
23 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
125 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
13 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
125 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
73.5 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
2.6     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
structure {in plan view}
  of palmetti type
lobes width
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in cross-section
presence
  present
shape
  tangentially-oval to tangentially-elliptic
tangentially length
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangential length TCt
22.6 µm    Mean (= average)
mean shape-ratio TCq
2.1     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
58 µm    Upper value of unspecified range (could be µ+s.d., but not known)
73 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
44 µm    Upper value of unspecified range (could be µ+s.d., but not known)
52 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
32.6 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.1     Mean (= average)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around tannin cells number
  one
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  thin-walled, slightly tapering, often rather similar to ends of normal hyphae, but mostly originating from a pseudoparenchyma (type O)
ramification presence-position
  absent
septa presence
  present
septa kind
  simple
diameter {proximal}
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
2.5 µm    Mean (= average)
3 µm    Maximum value
length
36 µm    Lower value of unspecified range (could be µ-s.d., but not known)
41 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  absent
cell wall thickness
0.2 µm    Mean (= average)
cell wall thickness {relative to mantle cells}
  thinner
cell wall evenness
  even in thickness
surface habit
  smooth
or    with soil particles

Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  plasmatically yellowish
pigment presence-distribution
  only distal
drops of exuded pigment presence
  present


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
2.5 µm    Mean (= average)
3 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    with crystals
or    without lens-shaped appositions
or    without spindle-shaped appositions
or    with soil particles
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness

Anatomy of the crystals occouring in emanating hyphae
presence shape
  irregularly shaped, amorphous
or    octahedral
orientation
  irregularly

Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  present
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  present
{of foreign origin} frequency
  consistently present
shape
  as normal hyphae
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  round
or    oval
diameter
1 µm    Mean (= average)
length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of siderophilous granulations
presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with anilin presence
  present
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  amyloid
amyloidity or dextrinoidity location
  all hyphae
amyloidity haustoria presence
  present
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with ruthenium-red presence
  absent
reaction with sulpho-vanillin presence
  absent

Ecology

soil pH
  pH 3 - 4
geographic occurrence continent
  North America

Tree

plant family
  Pinaceae
plant genus
  Tsuga
plant habitat kind
  forests, woods

Fungus

family
  Rhizopogonaceae
fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
195     Lower value of unspecified range (could be µ-s.d., but not known)
200     Upper value of unspecified range (could be µ+s.d., but not known)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
330     Lower value of unspecified range (could be µ-s.d., but not known)
340     Upper value of unspecified range (could be µ+s.d., but not known)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
140     Lower value of unspecified range (could be µ-s.d., but not known)
145     Upper value of unspecified range (could be µ+s.d., but not known)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
170     Lower value of unspecified range (could be µ-s.d., but not known)
175     Upper value of unspecified range (could be µ+s.d., but not known)

Remarks

public notes
  Inner mantle layers in plan view in the plectenchymatous nests hyphae 3-6 um in diam. and 5-15(-20) um long; cystidia plasmatically coloured; autofluorescence of mantle in section with UV-filter with outer two thirds to four fifths of mantle disctinctly blue, remaining parts greenish blue, with blue-filter distinctly green, with green-filter red; mantle in Melzer's reagent with amyloid granular cellular contents and with amyloid exuded drops, in FeSO4 pigment drops becoming slightly greyish-brown, in brillant-cresyl-blue walls slightly blue-green, in cotton-blue walls slightly blue, in anilin walls slightly reddish.

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