Russula acrifolia Romagn. + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R (1996) Russula acrifolia. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 105, Einhorn-Verlag, Schwäbisch Gmünd.
Agerer R, Franz F, Acker G (1994) The ectomycorrhizae of Russula acrifolia: an anatomical and ultrastructural treatise. Mycol Helvetica 6: 23-48.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• tips {per 10 mm} number
4     Lower value of unspecified range (could be µ-s.d., but not known)
6     Upper value of unspecified range (could be µ+s.d., but not known)
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
• main axis diameter
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.51 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  contact
Morphology of the unramified ends
• shape
  straight
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.9 mm    Upper value of unspecified range (could be µ+s.d., but not known)
5.2 mm    Maximum value
• diameter
0.33 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.45 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  white
• very tip colour
  whitish
• older parts colour
  grey
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
or    covered with soil particles
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
or    ochre, yellowish brown
or    whitish
• connection to mantle kind
  distinct
• origin location
  not specific
or    proximal
• margin habit
  hairy
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  gelatinous matrix between the hyphae (type C)
and    hyphae arranged net-like, with prominent cystidia (type D)
• matrix kind
  gelatinous
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  membranaceously brownish
• cell diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  colourless
or    membranaceously yellowish
or    membranaceously brownish
• cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
• cell contents presence-kind
  absent
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous with pseudoparenchymatous nests of cells
• matrix kind
  not gelatinous
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
30 µm    Minimum value
40 µm    Mean (= average)
50 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in longitudinal section
• presence
  present
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
105 µm    Maximum value
• radially diameter
3 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
73 µm    Mean (= average)
• mean shape-ratio TCq
7.1     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
17 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
115 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
13 µm    Minimum value
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
73 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.3     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• structure {in plan view}
  of palmetti type
• lobes width
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• middle mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic
• tangentially length
11 µm    Minimum value
23 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
• radially diameter
7 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
33 µm    Maximum value
• mean tangential length TCt
33.5 µm    Mean (= average)
• mean shape-ratio TCq
2.1     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
12 µm    Minimum value
22 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
37 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.2     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• septal pores configuration
  globular thickenings
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  flask-shaped with an apical knob (type D)
• apical knobs number
1     Minimum value
2     Mean (= average)
3     Maximum value
• apical knobs arrangement
  asymmetrically
or    serially in a bunch of two
• apical knobs shape
  ovoid
• apical knobs diameter
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
• apical knobs length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
• apical knobs wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs cell wall thickness {relative to cystidial body}
  thicker than those of cystidial body
• septa presence
  absent
• septa kind
  absent, with basal clamp only
• diameter {proximal}
4 µm    Minimum value
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• diameter {distal}
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• length
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
21 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  lacking or less intense
• cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness {relative to mantle cells}
  thicker
• cell wall evenness
  even in thickness
• surface habit
  smooth
• contents presence
  present
• contents type
  brownish, with latex-like droplets

Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  tortuous, screw-like
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  without lens-shaped appositions
or    without spindle-shaped appositions
or    with soil particles
• anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  differentiated; thick hyphae forming a core, septa complete or sometimes enlarged
(type E)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  absent
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
or    oval
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
2 µm    Mean (= average)
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome
• mantle matrix presence-kind
  of outer layers dark, of inner layers transparent

Autofluorescence

• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with anilin presence
  present
• reaction with cotton-blue-lactic-acid presence
  present [walls slightly blue]
• reaction with FeSO4 presence
  present
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  present
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with sulpho-vanillin presence
  present

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Russula sect. Compactae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mycorrhizal ends straight to slightly tortuous; autofluorescence of mantle in section with UV-filter greenish-blue and cystidia and inner mantle layers more intensely than middle parts, with blue-filter yellow, with green-filter red; mantle in sulfo-vanillin with distinctly grey walls and contents of some cystidia homogeneously grey, in KOH more intensely ochre, in guaiac blue, in FeSO4 slightly greenish, in cotton-blue walls slightly blue, in anilin weakly reddish to slightly ochre, in safranin red.

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