Arcangeliella borziana Cavara + Picea

– Item listing –

Literature

• references
  Egli S, Ayer F, Peter M, Schneider B (2001) Arcangeliella borziana Cavara + Picea abies (L.) Karst. Descr Ectomyc 5: 9-14.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate [often irregular and simple]
or    monopodial-pyramidal [often irregular and simple]
• tips {per 10 mm} number
  unknown
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  unknown
• main axis diameter
0.45 mm    Minimum value
0.6 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  contact
Morphology of the unramified ends
• mantle hydrophobicity presence
  unknown
• shape
  straight
or    bent [slightly]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.55 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  orange [red orange to dark salmon]
or    red [red orange to dark salmon]
• very tip colour
  unknown
• after injury colour
  unknown
• older parts colour
  brownish
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  unknown
• mantle secreted latex {after scratching mantle} colour-habit
  unknown
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  present
• location
  inner mantle layer
• shape
  unknown
• ramification presence-abundance
  unknown
• septa presence
  unknown
• contents colour
  colourless
• cell length
  unknown
• cell diameter
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
  unknown
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  unknown
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  unknown
• matrix location
  unknown
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells bearing a hyphal net (type P)
• hyphal net cells shape
  unknown
• hyphal net cells diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• hyphal net cell walls habit
  unknown
• hyphal net clamps presence
  absent
• matrix kind
  unknown
• hyphae arrangement
  not specific
• hyphae sticky matrix on hyphal surface presence
  unknown
• pores between cells presence
  unknown
• pores between cells abundance
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  unknown
• cell contents presence-kind
  unknown
• cell diameter
6 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
• cell length
6 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
• cell density
  unknown
• cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
• cell wall with globular thickenings
  absent
• cell wall projections presence
  unknown
• cell wall projections abundance
  unknown
• cell wall projections shape
  unknown
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  absent
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  unknown
• hyphae arrangement
  without pattern
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  unknown
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
  unknown
• cell contents presence-kind
  unknown
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
75 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
75 µm    Maximum value
• calyptra cell remnants presence
  unknown
• calyptra cell remnants type
  unknown
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• middle mantle layer presence
  not applicable
• middle mantle layer organisation
  not applicable
• middle mantle layer hyphae tangentially length
  not applicable
• middle mantle layer hyphae radially diameter
  not applicable
• inner mantle layer organisation
  plectenchymatous
• laticifers diameter
  unknown

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
  unknown
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
  unknown
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  thin-walled, slightly tapering, often rather similar to ends of normal hyphae, but mostly originating from a pseudoparenchyma (type O)
• branches ramification presence
  absent
• septa presence
  absent
• basal clamp walls abruptly thick
  absent
• foot cell presence
  absent
• diameter {proximal}
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter {distal}
  unknown
• length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  unknown
• cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
• cell wall thickness {relative to mantle cells}
  unknown
• cell wall evenness
  unknown
• surface habit
  smooth
• contents presence
  absent

Anatomy of cystidia (type 2)
• type
  fusiform (type F) [triangualr to fusiform]
• ramification presence-position
  absent
• septa presence
  present
• septa kind
  simple
• septa distance
  unknown
• septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• basal clamp walls abruptly thick
  absent
• foot cell presence
  absent
• diameter {proximal}
  unknown
• diameter {distal}
  unknown
• length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  unknown
• cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
• cell wall evenness
  unknown
• cell wall thickness {relative to mantle cells}
  unknown
• surface habit
  smooth
• contents presence
  absent
Anatomy of emanating hyphae
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  absent

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent

Chemical reactions

• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present [15%: after 10 min a very weak turquoise discoloration at the very tip]
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  present [carmine to wine-red, turning to dark-red]
• reaction with toluidin blue presence
  absent

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
385     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
325     Mean (= average)

Remarks

• public notes
  MboI: 260 bp, 200 bp, 125 bp, 100 bp; TaqI: 275 bp, 270 bp, 70 bp, 55 bp

– Item listing –