Hebeloma ammophilum Bohus + Fumana

– Item listing –

Literature

references
  Jakucs E, Magyar L, Beenken L (1999) Hebeloma ammophilum Bohus + Fumana procumbens (Dun.) Gr. Godr. Descr Ectomyc 4: 49-54.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
tips {per 10 mm} number
  unknown
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  unknown
main axis diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  short distance
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  bent
or    sinuous [slightly]
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
very tip colour
  ochre, yellowish brown
after injury colour
  unknown
older parts colour
  brownish
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  unknown
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  densely cottony
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
emanating elements cystidia location
  on outer mantle layer
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  gelatinous matrix between the hyphae (type C)
matrix kind
  gelatinous
hyphal system kind
  undifferentiated
hyphae short protrusions presence
  absent
hyphae sticky matrix on hyphal surface presence
  present
hyphae hyphal junctions angle {between}
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  present
septa clamps abundance
  infrequent
cell shape
  unknown
cell pigment location-colour
  absent
and    membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Mean (= average)
cell length
  unknown
cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
cell wall surface habit
  smooth
and    with many soil particles
drops of exuded pigment presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous [densely plectenchymatous to nearly pseudoparenchymatous]
matrix kind
  unknown
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
or    membranaceously yellowish [slightly]
cell diameter
3 µm    Mean (= average)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Mean (= average)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely, to nearly pseudoparenchymatous]
matrix kind
  unknown
hyphae arrangement
  without pattern
septa thickness
  unknown
septa clamps presence
  unknown
cell pigment location-colour
  absent
or    membranaceously yellowish
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Mean (= average)
cell length
  unknown
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
  unknown
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer presence
  present
middle mantle layer organisation
  plectenchymatous
middle mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  variable
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
10 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
16.8 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.5     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  periepidermal [at some places]
or    paraepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  infrequently lobed
lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
intrahyphal hyphae clamps presence
  unknown
septal pores configuration
  unknown
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
anastomoses surface habit
  smooth
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  Y-shaped
distance {from septum}
  one or two hyphal diam. below the septum
side-branches at septum number
  one side-branch at septum
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps surface kind
  smooth
clamps outline {in dorsal view}
  oval
clamps width {relative to hypha in dorsal view}
  as broad as
clamps outline {in lateral view}
  as a semicircle
clamps width {relative to hypha in lateral view}
  as broad as
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  infrequent
distribution
  unknown

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
anatomy emanating elements emanating hyphae cell diameter
3 µm    Mean (= average)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall layers number
  unknown
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent

Chemical reactions

reaction with FeSO4 presence
  absent
reaction with guaiac presence
  present [cells somewhat greenish]
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  absent [cells somewhat red]

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Cistaceae
plant genus
  Fumana
plant habitat kind
  dwarf shrub vegetation

Fungus

family
  Cortinariaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
320     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
190     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
170     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
360     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
340     Mean (= average)

Remarks

public notes
  EcoRI: 2x 350 bp; TaqI:340 bp, 300 bp, 60 bp

– Item listing –