Hebeloma ammophilum Bohus + Fumana

– Item listing –

Literature

• references
  Jakucs E, Magyar L, Beenken L (1999) Hebeloma ammophilum Bohus + Fumana procumbens (Dun.) Gr. Godr. Descr Ectomyc 4: 49-54.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• tips {per 10 mm} number
  unknown
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  unknown
• main axis diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  bent
or    sinuous [slightly]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
• very tip colour
  ochre, yellowish brown
• after injury colour
  unknown
• older parts colour
  brownish
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  unknown
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  densely cottony
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
• emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  gelatinous matrix between the hyphae (type C)
• matrix kind
  gelatinous
• hyphal system kind
  undifferentiated
• hyphae short protrusions presence
  absent
• hyphae sticky matrix on hyphal surface presence
  present
• hyphae hyphal junctions angle {between}
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  present
• septa clamps abundance
  infrequent
• cell shape
  unknown
• cell pigment location-colour
  absent
and    membranaceously yellowish
• cell contents presence-kind
  absent
• cell diameter
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Mean (= average)
• cell length
  unknown
• cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
• cell wall surface habit
  smooth
and    with many soil particles
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous [densely plectenchymatous to nearly pseudoparenchymatous]
• matrix kind
  unknown
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  colourless
or    membranaceously yellowish [slightly]
• cell diameter
3 µm    Mean (= average)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
  unknown
• cell contents presence-kind
  absent
• cell wall thickness
0.5 µm    Mean (= average)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [densely, to nearly pseudoparenchymatous]
• matrix kind
  unknown
• hyphae arrangement
  without pattern
• septa thickness
  unknown
• septa clamps presence
  unknown
• cell pigment location-colour
  absent
or    membranaceously yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Mean (= average)
• cell length
  unknown
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
  unknown
• calyptra cell remnants presence
  unknown
• calyptra cell remnants type
  unknown
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer presence
  present
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  variable
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
10 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
16.8 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.5     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  periepidermal [at some places]
or    paraepidermal
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  infrequently lobed
• lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• intrahyphal hyphae clamps presence
  unknown
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
• anastomoses surface habit
  smooth
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  Y-shaped
• distance {from septum}
  one or two hyphal diam. below the septum
• side-branches at septum number
  one side-branch at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps surface kind
  smooth
• clamps outline {in dorsal view}
  oval
• clamps width {relative to hypha in dorsal view}
  as broad as
• clamps outline {in lateral view}
  as a semicircle
• clamps width {relative to hypha in lateral view}
  as broad as
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  infrequent
• distribution
  unknown

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Mean (= average)
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent

Chemical reactions

• reaction with FeSO4 presence
  absent
• reaction with guaiac presence
  present [cells somewhat greenish]
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  absent [cells somewhat red]

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Cistaceae
• plant genus
  Fumana
• plant habitat kind
  dwarf shrub vegetation

Fungus

• family
  Cortinariaceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
320     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
190     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
170     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
360     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
340     Mean (= average)

Remarks

• public notes
  EcoRI: 2x 350 bp; TaqI:340 bp, 300 bp, 60 bp

– Item listing –