Paxillus involutus (Batsch) Fr. + Picea

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– Enlarged view –

Literature

• references
  Agerer R (1988) Studies on ectomycorrhizae XVII. The ontogeny of the ectomycorrhizal rhizomorphs of Paxillus involutus and Thelephora terrestris (Basidiomycetes). Nova Hedwigia 47: 311-334.
Agerer R, Gronbachs E (1989) Paxillus involutus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 27, Einhorn-Verlag, Schwäbisch Gmünd.
Franz F, Acker G (1995) Rhizomorphs of Picea abies ectomycorrhizae: Ultrastructural aspects and element analysis (EELS and ESI) on hyphal inclusions. Nova Hedwigia 60(1-2): 253-267.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibliotheca Mycologica 125: 1-216.
Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.

Morphology

Morphology of the mycorrhizal system
• length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
13 mm    Upper value of unspecified range (could be µ+s.d., but not known)
16 mm    Maximum value
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
or    abundant, dense
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.6 mm    Maximum value
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
• exploration type
  long distance
Morphology of the unramified ends
• shape
  straight
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Mean (= average)
0.4 mm    Maximum value
• colour
  brown
or    ochre, yellowish brown
or    white
• very tip colour
  whitish
• older parts colour
  dark brown
or    brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
• mantle surface {in detail} kind
  densely stringy
or    loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
• diameter
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  brown
or    brownish
• ramification kind-frequency
  frequently, at restricted points
or    infrequently, at restricted points
• connection to mantle kind
  distinct
• margin habit
  smooth
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  present
• abundance
  infrequent
• shape
  globular
• diameter
0.8 mm    Mean (= average)
• colour
  brown
• formation location
  laterally on rhizomorphs

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• matrix kind
  not gelatinous
• hyphal system kind
  undifferentiated
• hyphae hyphal junctions angle {between}
  ca. 45° and less
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  absent
• cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  colourless
• cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  present
• cell pigment location-colour
  absent
• cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• mantle different layers presence
  not discernable
• unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• unlayered mantle hyphae radially diameter
1 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
43 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
• radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
80.7 µm    Mean (= average)
• mean shape-ratio TCq
6.8     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
135 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
6 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
87.2 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
4     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section
• mantle different layers presence
  not discernible
• outer mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• unlayered mantle hyphae radially diameter
1 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  rectangular
• tangentially length
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
• radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangential length TCt
41.7 µm    Mean (= average)
• mean shape-ratio TCq
4.4     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
22 µm    Minimum value
26 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
6 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
31.2 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.5     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  two
or    three
or    several
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
or    two
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• septal pores configuration
  dolipore-like structures
• backwards-oriented ramifications presence
  present
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
• anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  fusiform (type F)
• septa presence
  absent
• septa kind
  absent, with basal clamp only
• diameter {proximal}
5 µm    Mean (= average)
• length
25 µm    Mean (= average)
• cell wall colour
  absent
• cell wall colour {relative to mantle cells}
  similar
• cell wall thickness {relative to mantle cells}
  similar in thickness
• surface habit
  smooth
• contents presence
  absent
• contents type
  absent

Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
• distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  infrequent
• distribution
  not specified

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
• anatomy emanating elements emanating hyphae cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
• nodia presence
  present
• internal nodia presence
  absent
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  present
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  filled with brownish or yellowish substances
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of sclerotia
• internal structure organisation
  pseudoparenchymatous with angular cells
• center structure habit
  compactly filled
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• {in foreign ectomycorrhizae} presence
  absent
• {of foreign origin} presence
  absent
• shape
  globular
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  present
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  present
• reaction with formol 40% presence
  present
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity haustoria presence
  absent
• reaction with phenole presence
  absent
• reaction with ruthenium-red presence
  present
• reaction with sulpho-vanillin presence
  present

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Paxillaceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mcorrhizal systems irregularly monopodial; mycorrhizal ends dirty-white, where air displaced by water yellow-brown or olive-brown, older parts yellow-brown or olive-brown; autofluorescence of mantle in section with UV-filter slightly greenish-blue, with blue-filter slightly ochre; mantle in sulfo-vanillin with slightly reddish-orange walls, in FeSO4 walls slightly greenish, in formol walls slightly greenish, in acid fuchsin walls slightly pink, in ruthenium-red walls slightly pink.

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