Paxillus involutus (Batsch) Fr. + Picea

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Literature

references
  Agerer R (1988) Studies on ectomycorrhizae XVII. The ontogeny of the ectomycorrhizal rhizomorphs of Paxillus involutus and Thelephora terrestris (Basidiomycetes). Nova Hedwigia 47: 311-334.
Agerer R, Gronbachs E (1989) Paxillus involutus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 27, Einhorn-Verlag, Schwäbisch Gmünd.
Franz F, Acker G (1995) Rhizomorphs of Picea abies ectomycorrhizae: Ultrastructural aspects and element analysis (EELS and ESI) on hyphal inclusions. Nova Hedwigia 60(1-2): 253-267.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibliotheca Mycologica 125: 1-216.
Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Research Publication no. 5. HMSO, London.

Morphology

Morphology of the mycorrhizal system
length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
13 mm    Upper value of unspecified range (could be µ+s.d., but not known)
16 mm    Maximum value
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  solitary or in small numbers
or    abundant, dense
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.6 mm    Maximum value
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
exploration type
  long distance
Morphology of the unramified ends
shape
  straight
or    tortuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Mean (= average)
0.4 mm    Maximum value
colour
  brown
or    ochre, yellowish brown
or    white
very tip colour
  whitish
older parts colour
  dark brown
or    brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
mantle surface {in detail} kind
  densely stringy
or    loosely stringy
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
diameter
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  brown
or    brownish
ramification kind-frequency
  frequently, at restricted points
or    infrequently, at restricted points
connection to mantle kind
  distinct
margin habit
  smooth
dimorphism presence
  absent
Morphology of sclerotia
presence
  present
abundance
  infrequent
shape
  globular
diameter
0.8 mm    Mean (= average)
colour
  brown
formation location
  laterally on rhizomorphs

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    cystidia
emanating elements cystidia location
  on outer mantle layer
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
matrix kind
  not gelatinous
hyphal system kind
  undifferentiated
hyphae hyphal junctions angle {between}
  ca. 45° and less
septa thickness {relative to cell walls}
  as thick as walls
cell shape
  cylindric, not constricted at septa
cell pigment location-colour
  absent
cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
matrix kind
  not gelatinous
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  present
cell pigment location-colour
  absent
cell diameter
3 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
mantle different layers presence
  not discernable
unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
unlayered mantle hyphae radially diameter
1 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
43 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
80.7 µm    Mean (= average)
mean shape-ratio TCq
6.8     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
135 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
6 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
87.2 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
4     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section
mantle different layers presence
  not discernible
outer mantle layer organisation
  plectenchymatous
unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
unlayered mantle hyphae radially diameter
1 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  rectangular
tangentially length
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangential length TCt
41.7 µm    Mean (= average)
mean shape-ratio TCq
4.4     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
22 µm    Minimum value
26 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
6 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
31.2 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.5     Mean (= average)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
anatomy mantle cross-section hyphal rows around tannin cells number
  two
or    three
or    several
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
or    two
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
septal pores configuration
  dolipore-like structures
backwards-oriented ramifications presence
  present
backwards-oriented clamps presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  fusiform (type F)
septa presence
  absent
septa kind
  absent, with basal clamp only
diameter {proximal}
5 µm    Mean (= average)
length
25 µm    Mean (= average)
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  similar
cell wall thickness {relative to mantle cells}
  similar in thickness
surface habit
  smooth
contents presence
  absent
contents type
  absent

Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  infrequent
distribution
  not specified

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
anatomy emanating elements emanating hyphae cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  present
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  present
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
contents presence-kind
  filled with brownish or yellowish substances
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of sclerotia
internal structure organisation
  pseudoparenchymatous with angular cells
center structure habit
  compactly filled
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
{in foreign ectomycorrhizae} presence
  absent
{of foreign origin} presence
  absent
shape
  globular
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  round
diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  present
reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  absent
reaction with cotton-blue-lactic-acid presence
  absent
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  present
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
amyloidity haustoria presence
  absent
reaction with phenole presence
  absent
reaction with ruthenium-red presence
  present
reaction with sulpho-vanillin presence
  present

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Paxillaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Mcorrhizal systems irregularly monopodial; mycorrhizal ends dirty-white, where air displaced by water yellow-brown or olive-brown, older parts yellow-brown or olive-brown; autofluorescence of mantle in section with UV-filter slightly greenish-blue, with blue-filter slightly ochre; mantle in sulfo-vanillin with slightly reddish-orange walls, in FeSO4 walls slightly greenish, in formol walls slightly greenish, in acid fuchsin walls slightly pink, in ruthenium-red walls slightly pink.

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