Russula ochroleuca (Pers.) Fr. + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R (1986) Studies on ectomycorrhizae III. Mycorrhizae formed by four fungi in the generea Lactarius and Russula on spruce. Mycotaxon 27: 1-59.
Agerer R (1987) Russula ochroleuca. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 1, Einhorn-Verlag, Schwäbisch Gmünd.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibliotheca Mycologica 125: 1-216.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest, a microscopical atlas. Kernforschungszentum, Karlsruhe.
Pillukat A (1991) Vergleichende Untersuchung der Ektomykorrhizen von Russula ochroleuca und Paxillus involutus an verschiedenen Baumarten. Dipl Thesis, Univ München.
Pillukat A, Agerer R (1992) Studien an Ektomykorrhizen XL. Vergleichende Untersuchungen zur baumbezogenen Variabilität der Ektomykorrhizen von Russula ochroleuca. Z Mykol 58(2): 211-242.

Morphology

Morphology of the mycorrhizal system
• length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
24 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
1.5 mm    Maximum value
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
or    present
• rhizomorphs frequency
  infrequent
• exploration type
  contact
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
or    tapering
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    yellow
or    green
• very tip colour
  yellowish
or    whitish
• older parts colour
  brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  yellowish or greenish
• mantle carbonizing presence
  present
• mantle surface {in general} habit
  smooth
• mantle surface {in detail} kind
  densely grainy or warty
or    loosely grainy or warty
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
• cross-section shape
  round or roundish
• colour
  yellow
• ramification kind-frequency
  frequently, at restricted points
• margin habit
  smooth
• dimorphism presence
  present
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells and mounds of flattened cells (type O)
• mound cells diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• mound cells granular contents
  present
• pores between cells presence
  absent
and    present
• pores between cells abundance
  infrequent
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell pigment location-colour
  absent
and    membranaceously yellowish
and    plasmatically yellowish
• cell diameter
2.5 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
• cell length
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell density
4     Minimum value
6     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
11     Maximum value
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
and    rough
and    with few crystals
• cell wall projections presence
  absent
• drops of exuded pigment presence
  present
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
or    pseudoparenchymatous
• matrix kind
  not gelatinous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  colourless
• cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
• cell length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
• cell length
7 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
49 µm    Maximum value
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
15     Minimum value
18     Lower value of unspecified range (could be µ-s.d., but not known)
23     Upper value of unspecified range (could be µ+s.d., but not known)
29     Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
20 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
54 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
2.5 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
24 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• outer mantle layer hyphae radially diameter
1.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
14 µm    Maximum value
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
14 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• inner mantle layer hyphae radially diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
22 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
125 µm    Maximum value
• radially diameter
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• mean tangenial length TCt
49 µm    Lower value of unspecified range (could be µ-s.d., but not known)
74.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
3.5     Lower value of unspecified range (could be µ-s.d., but not known)
5.5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
22 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
140 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
9 µm    Minimum value
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
54 µm    Lower value of unspecified range (could be µ-s.d., but not known)
89.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
2.6     Lower value of unspecified range (could be µ-s.d., but not known)
3.7     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
27 µm    Maximum value
• outer mantle layer hyphae radially diameter
1.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• middle mantle layer hyphae radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
23 µm    Maximum value
• inner mantle layer hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
13 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
55 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
• radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• mean tangential length TCt
29.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
31.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
1.1     Lower value of unspecified range (could be µ-s.d., but not known)
2.3     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  rectangular
or    round
or    radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
9 µm    Minimum value
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
13 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
21.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.9     Lower value of unspecified range (could be µ-s.d., but not known)
1.3     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• septal pores configuration
  globular thickenings
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses cell wall thickness {relative to remaining cell walls}
  thinner than
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
or    membranaceously yellowish
or    plasmatically yellowish
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
• distance {from septum}
  adjacent to septum

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
or    with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
or    ramified
• anatomy emanating elements emanating hyphae cell diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
or    differentiated; some hyphae very thick, which appear randomly distributed, pores of septa somtimes enlarged
(type D)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  absent
• {of foreign origin} presence
  absent
or    present
• {of foreign origin} frequency
  occasionally present
• shape
  globular
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
or    oval
• diameter
1.5 µm    Minimum value
2 µm    Mean (= average)
3 µm    Maximum value
• length
1.5 µm    Minimum value
2 µm    Mean (= average)
3 µm    Maximum value
Anatomy of siderophilous granulations
• presence
  absent
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome
• mantle matrix presence-kind
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  absent
or    present
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity haustoria presence
  absent
• reaction with phenole presence
  present
• reaction with phenole-aniline presence
  absent
• reaction with sulpho-vanillin presence
  absent
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Russula sect. Ingratae
• subsection
  Russula sect. Ingratae subsect. Felleinae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mycorrhizal systems irregularly monopodial-pyramidal to infrequently monopodial-pinnate; mycorrhizal ends sometimes slightly bent, dark yellow or yellow to yellowish-olive to olive-brown, older parts olive brown to brown to grey-brown or greyish-olive to grey-brown; middle mantle layers in plan view of a transitional type between pseudoparenchymatous and plectenchymatous; autofluorescenc of mantle in section with UV-filter in outer parts slightly greenish-yellow to yellowish-ochre, inner parts bueish-white, with blue-filter in outer parts yellow, inner parts bluish white, with green-filter slightly red throughout; mantle in KOH with red- to yellow-brown cell heaps, in brillant-cresyl-blue bright blue, after treatment with water blue, in toluidin-blue violet-blue, in cotton-blue cell walls slightly blue, in phenole dirty yellowish-green; nuclei lying close together.

– Item listing –