Pinirhiza rufomaculata + Pinus

– Item listing –

Literature

• references
  Wöllecke J, Münzenberger B, Hüttl RF (1998) "Pinirhiza rufomaculata" + Pinus sylvestris L.Descr Ectomyc 3: 73-78.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous [occasionally]
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.23 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.27 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent [extremely rare]
• exploration type
  contact
or    medium distance smooth
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
or    sinuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  white
or    whitish
• very tip colour
  white
• older parts colour
  unknown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  reddish
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
or    not smooth
• mantle surface {in detail} kind
  densely cottony
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
• emanating hyphae distribution
  lacking only at the tip
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.03 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  unknown
• colour
  whitish
or    white
• ramification kind-frequency
  unknown [not observed]
• connection to mantle kind
  distinct
• margin habit
  unknown
• dimorphism presence
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    emanating hyphae
• emanating elements cystidia location
  not applicable
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  unknown
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells bearing a hyphal net (type P)
• hyphal net cells shape
  not specialized
• hyphal net cells diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• hyphal net cell walls habit
  unknown
• matrix kind
  unknown
• hyphae arrangement
  as rosettes
• hyphae sticky matrix on hyphal surface presence
  unknown
• pores between cells presence
  unknown
• pores between cells abundance
  unknown
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously red [at patches]
• cell contents presence-kind
  unknown
• cell diameter
  unknown
• cell length
  unknown
• cell density
6     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
• cell wall projections presence
  absent
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous
• matrix kind
  unknown
• hyphae arrangement
  plectenchymatous, star-like
• cell shape
  polygonal
• cell pigment location-colour
  membranaceously red
• cell diameter
12.5 µm    Mean (= average)
• cell length
12.5 µm    Mean (= average)
• cell density
2     Minimum value
3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)
7     Maximum value
• cell contents presence-kind
  absent
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  pseudoparenchymatous
• matrix kind
  unknown
• hyphae arrangement
  star-like
• cell shape
  polygonal
• septa thickness
0.2 µm    Mean (= average)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
• septa clamps presence
  absent
• cell pigment location-colour
  unknown
• cell diameter
7.1 µm    Mean (= average)
• cell length
7.1 µm    Mean (= average)
• cell density
4     Minimum value
6     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
11     Maximum value
• cell contents presence-kind
  unknown
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle [without rosettes]
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
  unknown
• mantle different layers presence
  not discernable
• unlayered mantle hyphae tangentially length
  unknown
• unlayered mantle hyphae radially diameter
  unknown

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Mean (= average)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• radially diameter
6 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• mean tangenial length TCt
44 µm    Mean (= average)
• mean shape-ratio TCq
3.5     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  unknown
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
110 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
69 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.3     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
  unknown
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  unknown
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
  unknown
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section


Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  rectangular
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
11 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
49 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
2.4     Mean (= average)

Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  unknown
• intrahyphal hyphae clamps presence
  not applicable
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  not applicable
• anastomoses type
  closed by a simple septum, with a short bridge or bridge almost lacking (contact-septum)
• anastomoses surface habit
  smooth
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  unknown
• cell pigment location-colour
  absent
• cell contents presence-kind
  unknown
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
• distance {from septum}
  unknown
• side-branches at septum number
  unknown
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
10 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
  unknown
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
• internal nodia presence
  unknown
• gelatinous matrix presence
  unknown
• gelatinized hyphae presence
  unknown
• hyphae distinctly different diameter
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  unknown
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  unknown
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  unknown
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  unknown
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  unknown
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  unknown
• {in foreign ectomycorrhizae} abundance
  unknown
• {in foreign ectomycorrhizae} formed in fungal genus
  unknown
• {of foreign origin} presence
  absent

Autofluorescence

• mantle in section UV-filter 340-380 nm presence
  present [in all layers light bluish]

Chemical reactions

• reaction with acetic acid presence
  unknown
• reaction with acid fuchsin presence
  unknown
• reaction with AgNO3 presence
  unknown
• reaction with anilin presence
  unknown
• reaction with brillant-cresyl-blue presence
  unknown
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FEA presence
  unknown
• reaction with FeSO4 presence
  present [red patches dark brown]
• reaction with formol 40% presence
  present [red patches brown]
• reaction with guaiac presence
  absent
• reaction with HCl presence
  unknown
• reaction with KJ presence
  absent
• reaction with KOH 10% presence
  present [15%: emanating hyphae yellow]
• reaction with lactic acid presence
  present [after long incubation brown]
• reaction with Melzer's reagent presence
  dextrinoid [hyphae slightly red]
• amyloidity or dextrinoidity location
  emanating hyphae
• reaction with methylene blue presence
  unknown
• reaction with phenole presence
  unknown
• reaction with phenole-aniline presence
  unknown
• reaction with ruthenium-red presence
  unknown
• reaction with sudan III presence
  unknown
• reaction with sudan IV presence
  unknown
• reaction with sulpho-vanillin presence
  absent
• reaction with sulphuric acid presence
  unknown
• reaction with toluidin blue presence
  unknown
• reaction with trypan blue presence
  unknown
• reaction with water presence
  unknown

Ecology

• substrate
  in mineral soil
• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines

– Item listing –