Rhodocollybia butyracea + Pinus

– Item listing –

Literature

references
  Mleczko P (2004) Rhodocollybia butyracea (Bull.: Fr.) Lennox (forma butyracea) + Pinus sylvestris L. Decr Ectomyc 7/8: 101-108.

Morphology

Morphology of the mycorrhizal system
length
1.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  unknown
main axis diameter
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  unknown
exploration type
  short distance
Morphology of the unramified ends
mantle hydrophobicity presence
  unknown
shape
  straight
or    bent [sometimes]
shape {of distal end}
  tapering [slightly]
length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Mean (= average)
colour
  ochre, yellowish brown
or    whitish [whitish ochre]
very tip colour
  whitish
after injury colour
  unchanged
older parts colour
  brownish
or    ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  absent
or    present [partially]
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  loosely woolly
or    densely cottony [with fans]
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
emanating hyphae distribution
  unknown
Morphology of rhizomorphs
diameter
  unknown
cross-section shape
  unknown
colour
  unknown
after injury colour
  unchanged
ramification kind-frequency
  unknown
connection to mantle kind
  fan-like
origin location
  unknown
connection to substrate
  growing into the soil or organic layers
margin habit
  fan-like
dimorphism presence
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
emanating elements cystidia location
  generally present
or    on outer mantle layer
or    on rhizomorphs
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal system kind
  undifferentiated
hyphae short protrusions presence
  present
hyphae sticky matrix on hyphal surface presence
  unknown
hyphae hyphal junctions angle {between}
  unknown
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  absent
cell shape
  cylindric, constricted at septa
and    ampullate at both sides of septum [slightly]
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
75 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous [dense, resembling epidermoid pseudparenchyma]
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  membranaceously yellowish
cell diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
19.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
26 µm    Mean (= average)
26 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  not discernable
unlayered mantle organization
  plectenchymatous [compact]
unlayered mantle hyphae tangentially length
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
19 µm    Maximum value
unlayered mantle hyphae radially diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
  unknown
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
11.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
45 µm    Mean (= average)
mean shape-ratio TCq
2.6     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    round
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
11.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
41.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
16.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
38 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
23 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.85     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  in two rows, not reaching endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two [sometimes]
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  unknown
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
3.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
or    two [sometimes]
structure {in plan view}
  of palmetti type
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
intrahyphal hyphae clamps presence
  unknown
septal pores configuration
  globular thickenings
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a clamp, with a long bridge [? If anastomosis]
anastomoses surface habit
  smooth
anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  membranaceously yellowish
pigment presence-distribution
  unknown
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
or    Y-shaped [rarely]
distance {from septum}
  adjacent to septum
side-branches at septum number
  one side-branch at septum
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps surface kind
  smooth
clamps outline {in dorsal view}
  unknown
clamps width {relative to hypha in dorsal view}
  unknown
clamps outline {in lateral view}
  as a semicircle
clamps width {relative to hypha in lateral view}
  unknown
clamps hole presence
  present
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  absent

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  unknown
anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
75 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall layers number
  unknown
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
contents presence-kind
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [mantle bluish]
mantle in section blue-filter, 450-490 nm presence
  present [mantle greeish yellow]
mantle in section green-filter, 530-560 nm presence
  present [mantle reddish]

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present [cytoplasm and walls bluish]
reaction with ethanol 70% presence
  absent
reaction with guaiac presence
  present [mantle bluish]
reaction with KOH 10% presence
  absent [15%]
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with toluidin blue presence
  present [hyphal walls violet]

Ecology

substrate
  in organically enriched mineral horizon
soil pH
  pH 3 - 4
or    pH 4 - 5
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant subgenus-section
  two-needle-pines
plant habitat kind
  forests, woods

Fungus

family
  Tricholomataceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  HapII: 450 bp, 370 bp; HaeIII: 820 bp; EcoRI: 390 bp, 240 bp, 190 bp.

– Item listing –