Hygrophorus pustulatus (Pers.) Fr. + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Berg B, (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibliotheca Mycologica 125:1-217.
Gronbach E (1989) Hygrophorus pustulatus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 26, Einhorn-Verlag, Schwäbisch Gmünd

Morphology

Morphology of the mycorrhizal system
• length
2.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
20 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  medium distance smooth
Morphology of the unramified ends
• shape
  straight
or    bent
or    sinuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Minimum value
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  ochre, yellowish brown
• very tip colour
  yellowish
or    whitish
• older parts colour
  dark brown
or    brown
• mantle cortical cells visibility
  not visible
or    visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
Morphology of rhizomorphs
• diameter
0.02 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
• ramification kind-frequency
  infrequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  proximal
• margin habit
  smooth
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
and    pseudoparenchymatous
• mantle type
  epidermoid cells bearing a hyphal net (type Q)
• hyphal net cells shape
  aculeate, acuminated horn-shaped
and    triangular with obtuse ends
• hyphal net cells diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• hyphal net cell walls habit
  faint, delicate, inconspicuous, rather thin
and    distinct, rather thick
• pores between cells presence
  absent
• septa thickness {relative to cell walls}
  as thick as walls
• cell pigment location-colour
  absent
• cell diameter
3 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
• cell length
3 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• cell density
3     Minimum value
6     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• cell wall projections presence
  present
• cell wall projections abundance
  infrequent
• cell wall projections shape
  thicker at distal end
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
or    pseudoparenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, ring-like
• cell pigment location-colour
  colourless
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
200 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
or    ring-like
• septa clamps presence
  present
• cell pigment location-colour
  absent
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
10 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
200 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
15     Lower value of unspecified range (could be µ-s.d., but not known)
18     Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
13 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
23 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
35 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
145 µm    Maximum value
• radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangenial length TCt
90.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
96.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
5.4     Lower value of unspecified range (could be µ-s.d., but not known)
5.9     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
70.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
95.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• outer mantle layer hyphae radially diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
1 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
2     Mean (= average)
3     Maximum value
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
• radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangential length TCt
32.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
2.2     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm    Minimum value
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
24.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.3     Lower value of unspecified range (could be µ-s.d., but not known)
1.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
or    three
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• septal pores configuration
  globular thickenings
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a long bridge
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• distance {from septum}
  one or two hyphal diam. below the septum
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in lateral view}
  as a semicircle
or    less than a semicircle
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
or    present
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  abundant
• distribution
  partially crowded

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
22 µm    Minimum value
32 µm    Lower value of unspecified range (could be µ-s.d., but not known)
71 µm    Upper value of unspecified range (could be µ+s.d., but not known)
200 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
1.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as


Anatomy of rhizomorphs
• type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• internal nodia presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• shape
  globular
Anatomy of nuclei
• number {per cell}
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
• diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  present
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  present
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity haustoria presence
  absent
• reaction with phenole presence
  absent
• reaction with ruthenium-red presence
  present
• reaction with sulpho-vanillin presence
  present
• reaction with toluidin blue presence
  present

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Hygrophoraceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

• public notes
  Mycorrhizal ends flesh-coloured to light brown; autofluorescence of mantle in section with UV-filter with outer mantle portions yellowish-green, inner portions whitish-blue, with blue-filter outer mantle portions yellowish, inner portions ochre-yellowish, with green-filter mantle red throughout; mantle in sulfo-vanillin with slightly reddish walls and cytoplasm, in FeSO4 cytoplasm greyish green, in brillant-cresyl-blue walls violet, after rinsing with water blue, in toluidin-blue walls violet, in cotton-blue walls bluish, in acid fuchsin light pink, in ruthenium-red reddish.

– Item listing –