Hygrophorus pustulatus (Pers.) Fr. + Picea

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– Enlarged view –

Literature

references
  Berg B, (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibliotheca Mycologica 125:1-217.
Gronbach E (1989) Hygrophorus pustulatus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 26, Einhorn-Verlag, Schwäbisch Gmünd

Morphology

Morphology of the mycorrhizal system
length
2.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
20 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  medium distance smooth
Morphology of the unramified ends
shape
  straight
or    bent
or    sinuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Minimum value
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  ochre, yellowish brown
very tip colour
  yellowish
or    whitish
older parts colour
  dark brown
or    brown
mantle cortical cells visibility
  not visible
or    visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    smooth
Morphology of rhizomorphs
diameter
0.02 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  concolourous to mantle
ramification kind-frequency
  infrequently, at restricted points
connection to mantle kind
  distinct
origin location
  proximal
margin habit
  smooth
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
and    pseudoparenchymatous
mantle type
  epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  aculeate, acuminated horn-shaped
and    triangular with obtuse ends
hyphal net cells diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
hyphal net cell walls habit
  faint, delicate, inconspicuous, rather thin
and    distinct, rather thick
pores between cells presence
  absent
septa thickness {relative to cell walls}
  as thick as walls
cell pigment location-colour
  absent
cell diameter
3 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
cell length
3 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
cell density
3     Minimum value
6     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  infrequent
cell wall projections shape
  thicker at distal end
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
or    pseudoparenchymatous
hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, ring-like
cell pigment location-colour
  colourless
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
cell length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
200 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
or    ring-like
septa clamps presence
  present
cell pigment location-colour
  absent
cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
cell length
10 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
200 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
15     Lower value of unspecified range (could be µ-s.d., but not known)
18     Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
13 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
23 µm    Maximum value
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
middle mantle layer organisation
  plectenchymatous
middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
35 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
145 µm    Maximum value
radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
mean tangenial length TCt
90.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
96.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean shape-ratio TCq
5.4     Lower value of unspecified range (could be µ-s.d., but not known)
5.9     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Minimum value
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
70.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
95.9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
2 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
outer mantle layer hyphae radially diameter
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
middle mantle layer organisation
  plectenchymatous
middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
inner mantle layer hyphae radially diameter
1 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  present
rows number
2     Mean (= average)
3     Maximum value
shape
  tangentially-oval to tangentially-elliptic
tangentially length
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
mean tangential length TCt
32.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean shape-ratio TCq
2.2     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm    Minimum value
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
24.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.3     Lower value of unspecified range (could be µ-s.d., but not known)
1.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
or    three
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
septal pores configuration
  globular thickenings
backwards-oriented clamps presence
  present
anastomoses type
  open, with a long bridge
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
distance {from septum}
  one or two hyphal diam. below the septum
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in lateral view}
  as a semicircle
or    less than a semicircle
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent
or    present
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  abundant
distribution
  partially crowded

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
22 µm    Minimum value
32 µm    Lower value of unspecified range (could be µ-s.d., but not known)
71 µm    Upper value of unspecified range (could be µ+s.d., but not known)
200 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
1.5 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as


Anatomy of rhizomorphs
type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
internal nodia presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
shape
  globular
Anatomy of nuclei
number {per cell}
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
4     Maximum value
diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  present
reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
amyloidity haustoria presence
  absent
reaction with phenole presence
  absent
reaction with ruthenium-red presence
  present
reaction with sulpho-vanillin presence
  present
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Hygrophoraceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Mycorrhizal ends flesh-coloured to light brown; autofluorescence of mantle in section with UV-filter with outer mantle portions yellowish-green, inner portions whitish-blue, with blue-filter outer mantle portions yellowish, inner portions ochre-yellowish, with green-filter mantle red throughout; mantle in sulfo-vanillin with slightly reddish walls and cytoplasm, in FeSO4 cytoplasm greyish green, in brillant-cresyl-blue walls violet, after rinsing with water blue, in toluidin-blue walls violet, in cotton-blue walls bluish, in acid fuchsin light pink, in ruthenium-red reddish.

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