Russula sardonia Fr. + Pinus

– Item listing –

Literature

• references
  Mleczko P (2004) Russula sardonia Fr. + Pinus sylvestris L. Descr Ectomyc 7/8: 127-134. - Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
• length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous
or    coralloid
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  contact
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
• length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Minimum value
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  yellowish
or    reddish [rosy brown]
• very tip colour
  yellowish
or    reddish [light rosy brown]
• older parts colour
  brownish
or    yellow [dark]
or    orange [slightly]
or    reddish
• mantle cortical cells visibility
  unknown
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  whitish
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  densely grainy or warty
or    loosely grainy or warty
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of rhizomorphs
• diameter
  unknown
• cross-section shape
  unknown
• colour
  yellowish
• ramification kind-frequency
  unknown
• connection to mantle kind
  unknown
• origin location
  proximal
• margin habit
  unknown
• dimorphism presence
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    emanating hyphae
• emanating elements cystidia location
  unknown
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
• mantle type
  some cells containing droplets, staining in sulpho-vanillin; shape variable (type N)
and    epidermoid cells bearing a hyphal net (type Q)
• hyphal net cells shape
  aculeate, acuminated horn-shaped
and    with ring- or star-like structures
• hyphal net cells diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
• hyphal net cell walls habit
  distinct, rather thick
• hyphal net clamps presence
  absent
• hyphae arrangement
  not specific
• hyphae sticky matrix on hyphal surface presence
  absent
• pores between cells presence
  unknown
• pores between cells abundance
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell contents presence-kind
  droplets, that stain in sulpho-vanillin
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
• cell length
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• cell density
3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.3 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
• cell wall projections presence
  present
• cell wall projections abundance
  infrequent
• cell wall projections shape
  even in thickness
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  pseudoparenchymatous
• hyphae arrangement
  not applicable
or    plectenchymatous, without pattern
• cell shape
  epidermoid
• cell diameter
  not applicable
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
  not applicable
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell density
  not applicable
• cell contents presence-kind
  droplets, that stain in sulpho-vanillin
• cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
• cell wall surface habit
  smooth
• crystals diameter
  not applicable
• crystals length
  not applicable
• cell blue granules location
  not applicable
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [densely]
• hyphae arrangement
  without pattern
• hyphae vessel-like presence
  absent
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
  unknown
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
46 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• calyptra cell remnants presence
  unknown
• calyptra cell remnants type
  unknown
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• outer mantle layer hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• middle mantle layer hyphae radially diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  unknown
• inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
• inner mantle layer hyphae radially diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
  unknown
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [often triangular and flattened]
• tangentially length
11.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
5.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
30 µm    Mean (= average)
• mean shape-ratio TCq
1.94     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
11.5 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
59.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
52.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
31 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  unknown
• intrahyphal hyphae clamps presence
  absent
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  absent
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses surface habit
  unknown
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  wavy
or    tortuous
• cell pigment location-colour
  membranaceously yellowish
• pigment presence-distribution
  unknown
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  absent
• kind
  acute
or    Y-shaped
• distance {from septum}
  unknown
• side-branches at septum number
  one side-branch at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Minimum value
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
• internal nodia presence
  absent
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• hyphae distinctly different diameter
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  unknown
• {in foreign ectomycorrhizae} abundance
  unknown
• {in foreign ectomycorrhizae} formed in fungal genus
  unknown
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [mantle blue]
• mantle in section blue-filter, 450-490 nm presence
  present [mantle yellowish]
• mantle in section green-filter, 530-560 nm presence
  present [mantle red]

Chemical reactions

• reaction with acetic acid presence
  unknown
• reaction with acid fuchsin presence
  unknown
• reaction with AgNO3 presence
  unknown
• reaction with anilin presence
  unknown
• reaction with brillant-cresyl-blue presence
  unknown
• reaction with cotton-blue-lactic-acid presence
  present [cell walls blue]
• reaction with ethanol 70% presence
  unknown
• reaction with FEA presence
  unknown
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with HCl presence
  unknown
• reaction with KJ presence
  unknown
• reaction with KOH 10% presence
  absent [15%]
• reaction with lactic acid presence
  present [latex coagulating and eventually dissolving]
• reaction with Melzer's reagent presence
  absent
• reaction with methylene blue presence
  unknown
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  unknown
• reaction with sudan III presence
  absent
• reaction with sudan IV presence
  unknown
• reaction with sulpho-vanillin presence
  present [latex of gloeoplerous cells staining violet-black]
• reaction with sulphuric acid presence
  variable
• reaction with toluidin blue presence
  unknown
• reaction with trypan blue presence
  unknown
• reaction with water presence
  unknown

Ecology

• soil pH
  pH 3 - 4
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown ['9001' added to avoid duplicates!]#OCCURS TWICE IN SAME CHARACTER: PLEASE CORRECT DEFINITION!#

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Russula sect. Firmae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
380     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
350     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
120     Mean (= average)

Remarks

• public notes
  EcoR I: 520 bp, 350 bp; Taq I: 300 bp, 260 bp, 130 bp, 75 bp, 65 bp, 55 bp

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