Leucangium carthusianum (Tul.) Paol. + Pseudotsuga

– Item listing –

Literature

• references
  Palfner G, Agerer R (1998) Leucangium carthusianum (Tul.) Paol. (= Picoa carthusiana Tul. & Tu.l) + Pseudostuga menziesii (Mirb.) Franco. Descr Ectomyc 3: 37-42.

Morphology

Morphology of the mycorrhizal system
• length
6 mm    Lower value of unspecified range (could be µ-s.d., but not known)
16.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pyramidal [to irregularly ramified]
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs presence
  absent
• exploration type
  contact
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  bent
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown [reddish brown with occasional darker chestnut or purplish brown spots]
• very tip colour
  unknown
• older parts colour
  dark brown
• mantle cortical cells visibility
  visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  brown
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
or    covered with soil particles
• mantle surface {in detail} kind
  not applicable
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  unknown
• matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells (type L) [often also appearing as short, ramified or lobed hyphal cells, often in axially elongated chains]
• hyphae arrangement
  not specific
• hyphae sticky matrix on hyphal surface presence
  present
• pores between cells presence
  absent
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously brownish
• cell contents presence-kind
  absent
• cell diameter
4 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
56 µm    Upper value of unspecified range (could be µ+s.d., but not known)
62 µm    Maximum value
• cell density
3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)
6     Maximum value
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
and    with few soil particles
• cell wall projections presence
  unknown
• cell wall projections abundance
  unknown
• cell wall projections shape
  unknown
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous [outer portions mostly forming chains of cells in parallel to root-axis, cells of inner middle potions less regularly shaped]
• cell shape
  polygonal
or    roundish
or    epidermoid
• cell pigment location-colour
  membranaceously brownish
• cell diameter
4 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
39 µm    Upper value of unspecified range (could be µ+s.d., but not known)
47 µm    Maximum value
• cell density
  unknown
• cell contents presence-kind
  absent
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [hyphae short, irregularly shaped]
• hyphae arrangement
  without pattern
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  unknown
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
21 µm    Maximum value
• cell length
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33 µm    Upper value of unspecified range (could be µ+s.d., but not known)
48 µm    Maximum value
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not applicable
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
  unknown
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
5     Lower value of unspecified range (could be µ-s.d., but not known)
7     Upper value of unspecified range (could be µ+s.d., but not known)
8     Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
  unknown
• calyptra cell remnants presence
  present
• calyptra cell remnants type
  close to root cells
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
46 µm    Upper value of unspecified range (could be µ+s.d., but not known)
78 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Maximum value
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
47 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [flattened angular to spindle-like, sometimes isodiametrical]
• tangentially length
52 µm    Lower value of unspecified range (could be µ-s.d., but not known)
161 µm    Upper value of unspecified range (could be µ+s.d., but not known)
203 µm    Maximum value
• radially diameter
7 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
  unknown
• mean shape-ratio TCq
3.6     Lower value of unspecified range (could be µ-s.d., but not known)
7     Mean (= average)
13     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
33 µm    Lower value of unspecified range (could be µ-s.d., but not known)
190 µm    Upper value of unspecified range (could be µ+s.d., but not known)
233 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
76 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
  unknown
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.2     Lower value of unspecified range (could be µ-s.d., but not known)
3.5     Mean (= average)
7.5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  unknown
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section
• calyptra cell remnants presence
  present
• calyptra cell remnants kind
  close to root cells
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
29 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
• middle mantle layer organisation
  pseudoparenchymatous
• middle mantle layer hyphae tangentially length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
  unknown
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
22 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
39 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangential length TCt
  unknown
• mean shape-ratio TCq
0.6     Lower value of unspecified range (could be µ-s.d., but not known)
1.7     Mean (= average)
4.2     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  rectangular
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
36 µm    Lower value of unspecified range (could be µ-s.d., but not known)
67 µm    Upper value of unspecified range (could be µ+s.d., but not known)
100 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
17 µm    Minimum value
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
67 µm    Upper value of unspecified range (could be µ+s.d., but not known)
78 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
  unknown
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.6     Lower value of unspecified range (could be µ-s.d., but not known)
1     Mean (= average)
2.1     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  apparently one and a half row deep
• anatomy mantle cross-section hyphal cells around tannin cells shape
  unknown
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  unknown
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
• lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
• structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• intrahyphal hyphae clamps presence
  absent
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  absent
• anastomoses type
  unknown
• anastomoses surface habit
  unknown
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  unknown
• cell pigment location-colour
  membranaceously brownish
• pigment presence-distribution
  unknown
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  Y-shaped
• distance {from septum}
  unknown
• side-branches at septum number
  one side-branch at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  unknown
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
• anatomy emanating elements emanating hyphae cell diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  unknown
• {in foreign ectomycorrhizae} abundance
  unknown
• {in foreign ectomycorrhizae} formed in fungal genus
  unknown
• {of foreign origin} presence
  absent

Chemical reactions

• reaction with acetic acid presence
  unknown
• reaction with acid fuchsin presence
  absent
• reaction with AgNO3 presence
  unknown
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FEA presence
  unknown
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with HCl presence
  unknown
• reaction with KJ presence
  unknown
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with methylene blue presence
  unknown
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent
• reaction with sudan III presence
  unknown
• reaction with sudan IV presence
  unknown
• reaction with sulpho-vanillin presence
  present [whole mantle staining red, some hyphal septa separating into two and arching asymmtrically into cell lumen, thus forming o-like structures]
• reaction with sulphuric acid presence
  unknown
• reaction with toluidin blue presence
  unknown
• reaction with trypan blue presence
  unknown
• reaction with water presence
  unknown

Ecology

• geographic occurrence continent
  North America
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Pseudotsuga
• plant habitat kind
  forests, woods

Fungus

• family
  Helvellaceae

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
450     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
240     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
125     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
240     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
160     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
  variable [TaqI: 420 bp, 210 bp, 160 bp]
120     Mean (= average)

– Item listing –