Dermocybe huronensis (Ammirati & Smith) + Pinus

– Item listing –

Literature

• references
  Kuss P, Raidl S, Beenken L (2004) Cortinarius huronensis Ammirati & Smith var. huronensis + Pinus rotundata Link. Descr Ectomyc 7/8: 21-27.

Morphology

Morphology of the mycorrhizal system
• length
1 mm    Minimum value
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
8 mm    Maximum value
• ramification presence-type
  irregularly pinnate, dichotomous-like
• tips {per 10 mm} number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• ramification orders
0     Upper value of unspecified range (could be µ+s.d., but not known)
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Maximum value
• abundance
  nest-like, hyphal mats
or    abundant, dense
• main axis diameter
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Mean (= average)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
• exploration type
  medium distance fringe
Morphology of the unramified ends
• mantle hydrophobicity presence
  present
• shape
  straight
or    bent [slightly]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  yellowish
or    greenish [greenish to yellowish green]
or    green
• very tip colour
  yellowish
or    greenish
• after injury colour
  unknown
• older parts colour
  ochre, yellowish brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    covered with soil particles
or    not smooth
• mantle surface {in detail} kind
  loosely cottony
or    loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  unknown
• emanating hyphae distribution
  unknown
Morphology of rhizomorphs
• diameter
0.01 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.08 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• colour
  concolourous to mantle
or    yellowish
or    greenish
• after injury colour
  unknown
• ramification kind-frequency
  repeatedly into smaller filaments
• connection to mantle kind
  oblique
or    fan-like
• origin location
  not specific
• connection to substrate
  growing into the soil or organic layers
• margin habit
  hairy
or    woolly
• dimorphism presence
  present
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    emanating hyphae
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  unknown
• matrix location
  unknown
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  ring-like arrangement of hyphal bundles (type A)
and    hyphae rather irregularly arranged and no special pattern discernible (type B)
• clavate end cell cover presence
  absent
• matrix kind
  unknown
• hyphal system kind
  undifferentiated
• hyphae short protrusions presence
  absent
• hyphae sticky matrix on hyphal surface presence
  unknown
• hyphae hyphal junctions angle {between}
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  present
• septa clamps abundance
  secondary septa lacking
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  membranaceously yellowish
• cell contents presence-kind
  absent
• cell diameter
3.5 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
• cell length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous
• matrix kind
  unknown
• hyphae arrangement
  plectenchymatous, ring-like
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
3.5 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
• cell length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
• cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  unknown
• hyphae arrangement
  without pattern
• hyphae vessel-like presence
  absent
• septa thickness
  unknown
• septa clamps presence
  present
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
or    plectenchymatous
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
8 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• calyptra cell remnants presence
  present
• calyptra cell remnants type
  variable [frequent]
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer presence
  present
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
  unknown
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
65 µm    Maximum value
• radially diameter
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangenial length TCt
52.4 µm    Mean (= average)
• mean shape-ratio TCq
4.3     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
25 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
34 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.2     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
or    cylindrical
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type [irregularly lobed]
• lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  unknown
• intrahyphal hyphae clamps presence
  unknown
• septal pores configuration
  globular thickenings
• backwards-oriented ramifications presence
  present
• backwards-oriented clamps presence
  unknown
• anastomoses type
  closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
• anastomoses surface habit
  unknown
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  straight
or    wavy
• cell pigment location-colour
  membranaceously greenish
or    membranaceously yellowish
or    plasmatically yellowish
or    plasmatically greenish
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  unknown

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  slightly constricted
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts [infrequently]
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of the warts occouring on emanating hyphae
• presence
  present
• length
  unknown
Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
or    undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• internal nodia presence
  absent
• gelatinous matrix presence
  unknown
• gelatinized hyphae presence
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
or    only on peripheral hyphae
• contents presence-kind
  absent
• ampullate, trumpet-like inflated presence
  absent
• roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
or    only on peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
or    only on peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
or    only on peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
or    only on peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [bluish throughout]
• mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
• mantle in section green-filter, 530-560 nm presence
  present [reddish throughout]

Chemical reactions

• reaction with cotton-blue-lactic-acid presence
  present [hyphal walls and cytoplasm slightly blue]
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present [reddish brown]
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  not applicable [cytoplasm with dark brownish granula]
• reaction with phenole presence
  absent
• reaction with sulpho-vanillin presence
  present [walls slightly reddish]

Ecology

• substrate
  in peat
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Cortinariaceae
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
4     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
570     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
145     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
85     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 4. band from below number
55     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
420     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
350     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
120     Mean (= average)

Remarks

• public notes
  ECoRI: 500 bp, 390 bp; TaqI: 370 bp, 340 bp, 100 bp, 65 bp

– Item listing –