Russula alnetorum Romagn. + Alnus

– Item listing –

Literature

• references
  Beenken L (2001) Russula alnetorum Romagn. + Alnus viridis (Chaix) DC. Descr Ectomyc 5: 115-123.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
12 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate [rarely, mostly simple]
• tips {per 10 mm} number
  unknown
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  unknown
• main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent [very]
• exploration type
  medium distance smooth
Morphology of the unramified ends
• mantle hydrophobicity presence
  absent
• shape
  straight
or    bent [slightly]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  yellowish
• very tip colour
  unknown
• after injury colour
  unchanged
• older parts colour
  ochre, yellowish brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle dots presence-colour
  whitish [occasionally]
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely grainy or warty
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of rhizomorphs
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  cushion-shaped (semicircular)
• colour
  ochre, yellowish brown
• after injury colour
  unknown
• ramification kind-frequency
  infrequently, at restricted points
• connection to mantle kind
  distinct
• origin location
  proximal
• connection to substrate
  almost consistently growing along roots
• margin habit
  hairy
• dimorphism presence
  unknown
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
or    emanating hyphae
• emanating elements cystidia location
  not applicable
• crystals presence
  absent
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  surface net [on mantle surface]
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells, bearing mounds of roundish cells (type K)
and    angular cells and mounds of flattened cells (type O)
• mound cells shape
  triangular [a second type solitary, irreguarly elongate]
and    ovoid to cylindric
• mound cells diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mound cells length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
• mound cells cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mound cells cell wall kind
  smooth [verrucose, contents with droplets]
• mound cells granular contents
  absent
• clavate end cell cover presence
  absent
• matrix kind
  gelatinous
• hyphae arrangement
  not specific
• hyphae sticky matrix on hyphal surface presence
  present
• pores between cells presence
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell contents presence-kind
  droplets, that stain in sulpho-vanillin
• cell diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
• cell wall projections presence
  unknown
• cell wall projections abundance
  unknown
• cell wall projections shape
  unknown
• drops of exuded pigment presence
  absent
• roundish plasmatically yellow cells presence
  absent
• pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
• presence
  present
• organisation
  plectenchymatous [inner portions]
or    pseudoparenchymatous [outer portions]
• matrix kind
  not applicable
• hyphae arrangement
  plectenchymatous, without pattern
• cell shape
  polygonal
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
• cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [densely]
• matrix kind
  not applicable
• hyphae arrangement
  without pattern
• hyphae vessel-like presence
  absent
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  with oily droplets, which do not stain in sulpho-vanillin
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
10 µm    Mean (= average)
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• calyptra cell remnants presence
  present
• calyptra cell remnants type
  in two rows [up to three]
• mantle different layers presence
  not discernable
• unlayered mantle organization
  unknown
• unlayered mantle hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• unlayered mantle hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  paraepidermal
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  cylindrical
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
• intrahyphal hyphae clamps presence
  absent
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  absent
• anastomoses type
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
• cell pigment location-colour
  absent
or    membranaceously yellowish
• pigment presence-distribution
  unknown
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value


Anatomy of the warts occouring on emanating hyphae
• presence
  absent
Anatomy of rhizomorphs
• type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
• nodia presence
  absent
• internal nodia presence
  absent
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent
• hyphae distinctly different diameter
  absent
• cup-like structures on surface presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  absent
• contents presence-kind
  absent
• ampullate, trumpet-like inflated presence
  absent
• roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
• anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  absent
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
• anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  absent
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
• anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  absent
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
• anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  absent
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
• anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
• anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
• anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [yellow-green throughout]
• mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
• mantle in section green-filter, 530-560 nm presence
  present [red throughout]

Chemical reactions

• reaction with acetic acid presence
  present [walls slightly pink]
• reaction with cotton-blue-lactic-acid presence
  present [walls blue]
• reaction with ethanol 70% presence
  present [droplets dissolved]
• reaction with FeSO4 presence
  present [mantle olive]
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present [droplets dissovled]
• reaction with lactic acid presence
  present [droplets dissloved]
• reaction with Melzer's reagent presence
  absent
• reaction with sulpho-vanillin presence
  present [walls pink, contents of mantle cells with droplets becoming light brown]

Ecology

• substrate
  in mineral soil
• soil pH
  acid reaction
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Betulaceae
• plant genus
  Alnus
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• fruitbodies growth habit
  epigeous
or    pileate-smooth or wrinkled

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
500     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
240     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
50     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
370     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
330     Mean (= average)

Remarks

• public notes
  EcoRI: 390 bp, 320 bp, 50 bp, 40 bp; TaqI: 380 bp, 275 bp, 60 bp

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