Russula amoenolens Romagn. + Populus

– Item listing –

Literature

references
  Jakucs E, Beenken L (1999) Russula amoenolensRomagn. + Populus alba L. Descr Ectomyc 4: 115-119.

Morphology

Morphology of the mycorrhizal system
length
4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate [irregularly]
or    monopodial-pyramidal [irregularly]
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  abundant, dense
main axis diameter
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  short distance
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  straight
or    bent [slightly]
shape {of distal end}
  tapering [slightly]
length
1 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  white
very tip colour
  yellowish
after injury colour
  unchanged
older parts colour
  ochre, yellowish brown [light]
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  densely short-spiny
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of rhizomorphs
diameter
0.01 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.02 mm    Upper value of unspecified range (could be µ+s.d., but not known)
connection to substrate
  growing into the soil or organic layers
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
or    cystidia
emanating elements cystidia location
  on outer mantle layer
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
clavate end cell cover presence
  absent
matrix kind
  not gelatinous
hyphae short protrusions presence
  unknown
hyphae sticky matrix on hyphal surface presence
  present
hyphae hyphal junctions angle {between}
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell shape
  cylindric, not constricted at septa
cell pigment location-colour
  absent
and    membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
cell diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Mean (= average)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  without pattern [at places arranged in parallel]
hyphae vessel-like presence
  absent
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer presence
  present
middle mantle layer organisation
  unknown
middle mantle layer hyphae tangentially length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  unknown
inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
inner mantle layer hyphae radially diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
40 µm    Minimum value
55 µm    Lower value of unspecified range (could be µ-s.d., but not known)
65 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
14.6 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.25     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  paraepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
structure {in plan view}
  infrequently lobed
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  unknown
intrahyphal hyphae clamps presence
  absent
septal pores configuration
  unknown
backwards-oriented ramifications presence
  present
backwards-oriented clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  flask-shaped with an apical knob (type D)
apical knobs number
1     Mean (= average)
apical knobs arrangement
  symmetrically
apical knobs shape
  globose
apical knobs diameter
  unknown
apical knobs length
  unknown
apical knobs wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
apical knobs cell wall thickness {relative to cystidial body}
  as thick as those of cystidial body
septa presence
  present [occasionally]
septa kind
  simple
septa distance {between}
  unknown
septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
0     Upper value of unspecified range (could be µ+s.d., but not known)
1     Maximum value
foot cell presence
  absent
diameter {proximal}
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
  unknown
length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  unknown
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  similar in thickness
cell wall evenness
  unknown
surface habit
  smooth
contents presence
  absent

Anatomy of cystidia (type 2)
type
  bottle-shaped with a straight neck (type B)
ramification presence-position
  absent
septa presence
  present [occasionally]
septa kind
  simple
septa distance
  unknown
septa number
0     Upper value of unspecified range (could be µ+s.d., but not known)
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Maximum value
basal clamp walls abruptly thick
  absent
foot cell presence
  absent
diameter {proximal}
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
  unknown
length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  similar
cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
cell wall evenness
  unknown
cell wall thickness {relative to mantle cells}
  similar in thickness
surface habit
  smooth
contents presence
  absent
Anatomy of emanating hyphae
shape
  unknown
cell pigment location-colour
  absent
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  Y-shaped
distance {from septum}
  one or two hyphal diam. below the septum
side-branches at septum number
  unknown
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    with soil particles
anatomy emanating elements emanating hyphae cell wall surface structures shape
  variable
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of the warts occouring on emanating hyphae
presence
  absent
Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [mantle distinctly yellowish brown, cystidia bluish]
mantle in section blue-filter, 450-490 nm presence
  present [mantle distinctly yellowish brown, cystidia yellowish]
mantle in section green-filter, 530-560 nm presence
  present [reddish]

Chemical reactions

reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  dextrinoid
amyloidity or dextrinoidity location
  matrix [on mantle surface]
reaction with sulpho-vanillin presence
  absent

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Salicaceae
plant genus
  Populus
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Russula sect. Ingratae
subsection
  Russula sect. Ingratae subsect. Foetentinae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
500     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
220     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
340     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
260     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
115     Mean (= average)

Remarks

public notes
  EcoRI: 390bp, 330 bp; TaqI: 290 bp, 280 bp, 95 bp, 62 bp

– Item listing –