Rhizopogon vulgaris (Vitt.) M. Lange + Pinus

– Item listing –

Literature

references
  Jakucs E, Bratek Z, Beenken L, Agerer R (1998) Rhizopogon vulgaris (Vitt.) M. Lange var. intermedius Svrcek + Pinus nigra Arn. Descr Ectomyc 3: 111-116.

Morphology

Morphology of the mycorrhizal system
length
2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
or    coralloid
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  unknown
main axis diameter
  unknown
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  unknown
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  unknown
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
exploration type
  long distance
Morphology of the unramified ends
mantle hydrophobicity presence
  unknown
shape
  unknown
shape {of distal end}
  not inflated, cylindric
length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  red [pink]
or    lilac, light reddish blue [pink]
very tip colour
  reddish [whitish to light pink]
or    whitish [whitish to light pink]
older parts colour
  red [darker pink with red-purple patches]
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  not applicable
mantle secreted latex {after scratching mantle} colour-habit
  not applicable
mantle dots presence-colour
  reddish [darker pink with red-purple patches]
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
or    covered with soil particles [covered with a layer of crystals and of soil particles]
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish [oval to dorsiventrally flattened]
colour
  red [pink, at older parts with red-purple patches]
or    white
ramification kind-frequency
  frequently, at restricted points
connection to mantle kind
  distinct
origin location
  unknown
margin habit
  smooth [thick rhizomorphs smooth, covered densely with crystals and soil particles]
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
emanating elements cystidia location
  not applicable
crystals presence
  present
crystals location
  outer mantle layer
or    peripheral hyphae of rhizomorphs
blue granules presence
  unknown
blue granules location
  unknown
Anatomy of laticifers
presence
  absent
location
  not applicable
shape
  not applicable
ramification presence-abundance
  not applicable
septa presence
  not applicable
contents colour
  not applicable
cell length
  not applicable
cell diameter
  not applicable
cell wall thickness
  not applicable
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  gelatinous matrix between the hyphae (type C)
hyphal net cells shape
  not applicable
hyphal net cells diameter
  not applicable
hyphal net cell walls habit
  not applicable
mound cells diameter
  not applicable
matrix kind
  gelatinous
hyphal system kind
  undifferentiated
hyphae arrangement
  not applicable
hyphae short protrusions presence
  unknown
hyphae sticky matrix on hyphal surface presence
  present
pores between cells presence
  not applicable
pores between cells abundance
  not applicable
hyphae hyphal junctions angle {between}
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
septa clamps abundance
  not applicable
cell shape
  unknown
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
  unknown
cell length
  unknown
cell density
  not applicable
cell wall thickness
0.5 µm    Mean (= average)
cell wall surface habit
  with many crystals
and    with many soil particles
cell wall projections presence
  not applicable
cell wall projections abundance
  not applicable
cell wall projections shape
  not applicable
crystals diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
crystals length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
drops of exuded pigment presence
  unknown
pigment granula on surface presence
  absent
blue granules location
  unknown
Anatomy of the middle mantle layer
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  plectenchymatous, without pattern
cell shape
  not applicable
cell pigment location-colour
  membranaceously yellowish
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell density
  not applicable
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Mean (= average)
cell wall surface habit
  smooth
crystals diameter
  not applicable
crystals length
  not applicable
cell blue granules location
  unknown
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  without pattern
cell shape
  not applicable
septa thickness
  unknown
septa clamps presence
  unknown
cell pigment location-colour
  membranaceously yellowish
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell density
  not applicable
cell contents presence-kind
  absent
cell blue granules location
  unknown
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
30 µm    Mean (= average)
calyptra cell remnants presence
  present
calyptra cell remnants type
  close to root cells
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer organisation
  plectenchymatous
middle mantle layer hyphae tangentially length
22 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
inner mantle layer hyphae radially diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
unlayered mantle hyphae tangentially length
  not applicable
unlayered mantle hyphae radially diameter
  not applicable
laticifers diameter
  not applicable

Anatomy of the tannin cells in longitudinal section
presence
  present [solitary at parts below the ramification]
rows number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  unknown
tangentially length
  unknown
radially diameter
  unknown
mean tangenial length TCt
  unknown
mean shape-ratio TCq
  unknown

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  unknown
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
32.6 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.81     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  unknown
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
  unknown
anatomy mantle longitudinal section hyphal rows around tannin cells number
  unknown
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
intrahyphal hyphae clamps presence
  not applicable
septal pores configuration
  unknown
backwards-oriented ramifications presence
  absent
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses surface habit
  unknown
anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  unknown
cell pigment location-colour
  unknown
pigment presence-distribution
  unknown
cell contents presence-kind
  unknown
drops of exuded pigment presence
  present

Anatomy of the ramification of emanating hyphae
presence
  unknown
kind
  unknown
distance {from septum}
  unknown
side-branches at septum number
  unknown
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
clamps presence
  absent
clamps surface kind
  unknown
clamps outline {in dorsal view}
  unknown
clamps width {relative to hypha in dorsal view}
  unknown
clamps outline {in lateral view}
  less than a semicircle
clamps width {relative to hypha in lateral view}
  as broad as
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  not applicable
abundance
  not applicable
distribution
  not applicable

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  unknown
anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
anatomy emanating elements emanating hyphae cell diameter
  unknown
anatomy emanating elements emanating hyphae cell length
  unknown
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    with crystals
or    with soil particles
anatomy emanating elements emanating hyphae cell wall surface structures shape
  not applicable
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall layers number
  unknown
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown

Anatomy of the crystals occouring in emanating hyphae
presence shape
  cuboid
or    octahedral
orientation
  unknown
diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the warts occouring on emanating hyphae
presence
  not applicable
length
  not applicable
Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  present
internal nodia presence
  absent
gelatinous matrix presence
  present [on surface]
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  unknown
conical young side-branches presence
  unknown
a "ball" of intertwined, ramified, thin hyphae presence
  unknown
blue granules location
  unknown

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  unknown
contents presence-kind
  filled with brownish or yellowish substances [some vessel-like hyphae]
ampullate, trumpet-like inflated presence
  absent [vessel-like hyphae somtimes lightly ampullate at septa]
ampullate inflations thickness
  not applicable
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  unknown
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances [some vessel-like hyphae]
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent [vessel-like hyphae somtimes lightly ampullate at septa]
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
  not applicable
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  unknown
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances [some vessel-like hyphae]
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent [vessel-like hyphae somtimes lightly ampullate at septa]
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
  not applicable
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  unknown
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances [some vessel-like hyphae]
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent [vessel-like hyphae somtimes lightly ampullate at septa]
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
  not applicable
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  unknown
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances [some vessel-like hyphae]
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent [vessel-like hyphae somtimes lightly ampullate at septa]
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
  not applicable
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of ectomycorrhiza former} abundance
  not applicable
{in foreign ectomycorrhizae} presence
  unknown
{in foreign ectomycorrhizae} abundance
  unknown
{in foreign ectomycorrhizae} formed in fungal genus
  unknown
{of foreign origin} presence
  absent
{of foreign origin} frequency
  not applicable
{of foreign origin} formed by genus
  not applicable
shape
  not applicable
diameter
  not applicable
Anatomy of nuclei
number {per cell}
  unknown
shape
  unknown
diameter
  unknown
length
  unknown
distance {between each other}
  unknown
Anatomy of siderophilous granulations
presence
  unknown
frequency
  unknown
arrangement
  unknown
diameter
  unknown
Ultrastructure
septal pores type
  unknown
Woronin bodies shape
  not applicable
hyphae warts shape
  not applicable
mantle matrix presence-kind
  unknown

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [light blue]
mantle in section blue-filter, 450-490 nm presence
  present [yellow-green]
mantle in section green-filter, 530-560 nm presence
  present [strongly red]

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with anilin presence
  present [light brown]
reaction with brillant-cresyl-blue presence
  absent
reaction with cotton-blue-lactic-acid presence
  absent
reaction with ethanol 70% presence
  unknown
reaction with FeSO4 presence
  absent
reaction with formol 40% presence
  unknown
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
amyloidity or dextrinoidity location
  not applicable
amyloidity haustoria presence
  not applicable
reaction with phenole presence
  unknown
reaction with phenole-aniline presence
  unknown
reaction with ruthenium-red presence
  unknown
reaction with sudan III presence
  unknown
reaction with sulpho-vanillin presence
  absent [sulphate crystals on surface light red]
reaction with toluidin blue presence
  unknown
reaction with water presence
  unknown
chemical compounds type
  unknown

Ecology

substrate
  unknown
{occurence in} humus type
  unknown
soil pH
  pH 6 - 7
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown ['9001' added to avoid duplicates!]#OCCURS TWICE IN SAME CHARACTER: PLEASE CORRECT DEFINITION!#

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant habitat kind
  forests, woods

Fungus

family
  Rhizopogonaceae
fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
405     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
290     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
55     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
4     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
240     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
220     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
145     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 4. band from below number
115     Mean (= average)

Remarks

public notes
  Taq I: 295 bp, 110 bp, 90 bp, 87 bp, 64 bp

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