Russula densifolia Secr. ex Gill. + Picea

– Item listing –

Literature

• references
  Beenken L (2001) Russula densifolia Secr. ex Gill. + Picea abies (L.) H. Karst. Descr Ectomyc 5: 157-161.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, University of München. http://edoc.ub.uni-muenchen.de/archive/00003175/

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
21 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  absent
or    monopodial-pinnate
• tips {per 10 mm} number
  unknown
• ramification orders
  unknown
• abundance
  unknown
• main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• taste {after 2-3 min chewing}
  unknown
• rhizomorphs presence
  absent
• rhizomorphs frequency
  not applicable
• exploration type
  contact
Morphology of the unramified ends
• mantle hydrophobicity presence
  present [partly]
• shape
  straight
or    bent [slightly bent]
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  ochre, yellowish brown [ochre to light brown]
• very tip colour
  ochre, yellowish brown [ochre to light brown (lighter than other parts)]
• older parts colour
  unknown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  unknown
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  not applicable
• mantle secreted latex {after scratching mantle} colour-habit
  not applicable
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    covered with soil particles
or    not smooth
• mantle surface {in detail} kind
  densely short-spiny [velvety]
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  unknown
Morphology of rhizomorphs
• diameter
  not applicable
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  emanating hyphae
or    cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  absent
• crystals location
  not applicable
• blue granules presence
  absent
• blue granules location
  not applicable
Anatomy of laticifers
• presence
  absent
• location
  not applicable
• shape
  not applicable
• ramification presence-abundance
  not applicable
• septa presence
  not applicable
• contents colour
  not applicable
• cell length
  not applicable
• cell diameter
  not applicable
• cell wall thickness
  not applicable
Anatomical features of the mantle
• completeness
  complete
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
• hyphal net cells shape
  not applicable
• hyphal net cells diameter
  not applicable
• hyphal net cell walls habit
  not applicable
• mound cells diameter
  not applicable
• matrix kind
  gelatinous
• hyphal system kind
  undifferentiated
• hyphae arrangement
  not applicable
• hyphae short protrusions presence
  unknown
• hyphae sticky matrix on hyphal surface presence
  unknown
• pores between cells presence
  not applicable
• pores between cells abundance
  not applicable
• hyphae hyphal junctions angle {between}
  unknown
• septa thickness {relative to cell walls}
  unknown
• septa clamps presence
  absent
• septa clamps abundance
  not applicable
• cell shape
  unknown
• cell pigment location-colour
  absent
and    membranaceously yellowish
• cell contents presence-kind
  absent
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
  unknown
• cell density
  not applicable
• cell wall thickness
0.3 µm    Mean (= average)
• cell wall surface habit
  smooth
and    with few soil particles
• cell wall projections presence
  not applicable
• cell wall projections abundance
  not applicable
• cell wall projections shape
  not applicable
• crystals diameter
  not applicable
• crystals length
  not applicable
• drops of exuded pigment presence
  absent
• pigment granula on surface presence
  absent
• blue granules location
  within cells
Anatomy of the middle mantle layer
• organisation
  plectenchymatous [loosely to densely]
• matrix kind
  gelatinous
• hyphae arrangement
  plectenchymatous, without pattern
• cell shape
  not applicable
• cell pigment location-colour
  colourless
or    membranaceously yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• cell length
2 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell density
  not applicable
• cell contents presence-kind
  absent
• cell wall thickness
0.3 µm    Mean (= average)
• cell wall surface habit
  smooth
• crystals diameter
  not applicable
• crystals length
  not applicable
• cell blue granules location
  not applicable
Anatomy of the inner mantle layer
• organisation
  plectenchymatous [densely]
• matrix kind
  unknown
• hyphae arrangement
  without pattern
• cell shape
  not applicable
• septa thickness
  unknown
• septa clamps presence
  absent
• cell pigment location-colour
  absent
or    plasmatically yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell density
  not applicable
• cell contents presence-kind
  absent
• cell blue granules location
  not applicable
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
30 µm    Mean (= average)
• calyptra cell remnants presence
  present
• calyptra cell remnants type
  close to root cells
• mantle different layers presence
  discernable [two]
• outer mantle layer organisation
  plectenchymatous [loosely, (7)10-20 µm wide]
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  not applicable
• middle mantle layer hyphae tangentially length
  not applicable
• middle mantle layer hyphae radially diameter
  not applicable
• inner mantle layer organisation
  plectenchymatous [densely]
• inner mantle layer hyphae tangentially length
1.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• unlayered mantle hyphae tangentially length
  not applicable
• unlayered mantle hyphae radially diameter
  not applicable
• laticifers diameter
  not applicable

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [flattened]
• tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
  unknown
• mean shape-ratio TCq
  unknown

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular [parallel to the root surface]
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
  unknown
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
  unknown

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
• anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
• structure {in plan view}
  of palmetti type
• lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  unknown
• intrahyphal hyphae clamps presence
  not applicable
• septal pores configuration
  unknown
• backwards-oriented ramifications presence
  unknown
• backwards-oriented clamps presence
  absent
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses surface habit
  smooth
• anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
• anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
• anastomoses location
  unknown
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  flask-shaped with an apical knob (type D)
• apical knobs number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs arrangement
  solitary, not in chains [often solitary and not in a chain]
or    asymmetrically
or    serially in a bunch of two
• apical knobs shape
  ovoid
• apical knobs diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs wall thickness
0.3 µm    Mean (= average)
0.5 µm    Maximum value
• apical knobs cell wall thickness {relative to cystidial body}
  as thick as those of cystidial body
• ramification presence-position
  absent
• ramification type
  not applicable
• branches ramification presence
  not applicable
• septa presence
  present [infrequently with one simple septum]
• septa kind
  simple
• septa distance {between}
  unknown
• septa number
  not applicable
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Maximum value
• foot cell presence
  absent
• diameter {proximal}
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter {distal}
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• cell wall colour
  absent
or    yellowish
• cell wall colour {relative to mantle cells}
  similar
• cell wall thickness
0.3 µm    Mean (= average)
0.5 µm    Maximum value
• cell wall thickness {relative to mantle cells}
  thicker [indistinctly thicker]
• cell wall evenness
  unknown
• surface habit
  smooth
• contents presence
  present
• contents type
  brownish, with latex-like droplets

Anatomy of cystidia (type 2)
• type
  not applicable
• apical knobs shape
  oval
• apical knobs diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• apical knobs length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
or    membranaceously yellowish
• pigment presence-distribution
  unknown
• cell contents presence-kind
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  unknown
• distance {from septum}
  unknown
• side-branches at septum number
  unknown
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
• clamps presence
  absent
• clamps surface kind
  not applicable
• clamps outline {in dorsal view}
  not applicable
• clamps width {relative to hypha in dorsal view}
  not applicable
• clamps outline {in lateral view}
  not applicable
• clamps width {relative to hypha in lateral view}
  not applicable
• clamps hole presence
  not applicable
• clamps blister-like structure {at basis} presence
  not applicable

Anatomy of secondary septa of emanating hyphae
• presence
  not applicable
• abundance
  not applicable
• distribution
  not applicable

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell length
  unknown
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  not applicable
• anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Mean (= average)
• anatomy emanating elements emanating hyphae cell wall layers number
  unknown
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown

Anatomy of the crystals occouring in emanating hyphae
• presence shape
  not applicable
• orientation
  not applicable
• diameter
  not applicable
• length
  not applicable

Anatomy of the warts occouring on emanating hyphae
• presence
  not applicable
• length
  not applicable
Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
• contents presence-kind
  not applicable
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  not applicable
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  not applicable
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  not applicable
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae contents presence-kind
  not applicable
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of ectomycorrhiza former} abundance
  not applicable
• {in foreign ectomycorrhizae} presence
  unknown
• {in foreign ectomycorrhizae} abundance
  unknown
• {in foreign ectomycorrhizae} formed in fungal genus
  unknown
• {of foreign origin} presence
  absent
• {of foreign origin} frequency
  not applicable
• {of foreign origin} formed by genus
  not applicable
• shape
  not applicable
• diameter
  not applicable
Anatomy of nuclei
• number {per cell}
  unknown
• shape
  unknown
• diameter
  unknown
• length
  unknown
• distance {between each other}
  unknown
Anatomy of siderophilous granulations
• presence
  unknown
• frequency
  unknown
• arrangement
  unknown
• diameter
  unknown
Ultrastructure
• septal pores type
  unknown
• Woronin bodies shape
  not applicable
• hyphae warts shape
  not applicable
• mantle matrix presence-kind
  unknown

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present [yellow-green througout]
• mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
• mantle in section green-filter, 530-560 nm presence
  present [red throughout]

Chemical reactions

• reaction with acid fuchsin presence
  present [walls slightly pink]
• reaction with anilin presence
  unknown
• reaction with brillant-cresyl-blue presence
  unknown
• reaction with cotton-blue-lactic-acid presence
  present [walls dark blue]
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  unknown
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• amyloidity or dextrinoidity location
  not applicable
• amyloidity haustoria presence
  not applicable
• reaction with phenole presence
  unknown
• reaction with phenole-aniline presence
  unknown
• reaction with ruthenium-red presence
  unknown
• reaction with sudan III presence
  unknown
• reaction with sulpho-vanillin presence
  present [walls pink, contents of a few cystidia becoming dark brown]
• reaction with toluidin blue presence
  unknown
• reaction with water presence
  unknown
• chemical compounds type
  unknown

Ecology

• substrate
  in organic layer
• {occurence in} humus type
  unknown
• soil pH
  acid reaction
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown ['9001' added to avoid duplicates!]#OCCURS TWICE IN SAME CHARACTER: PLEASE CORRECT DEFINITION!#

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Russulaceae
• subgenus-section
  Russula sect. Compactae
• subsection
  not applicable
• fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

• PCR- ITS1, -ITS4 restriction enzyme Alu bands number
2     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
670     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
220     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
370     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
370     Mean (= average)
• PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
120     Mean (= average)

Remarks

• public notes
  Beenken L (2001) Russula densifolia Secr. Ex Gill. + Picea abies (L.) H. Karst. Descr Ectomyc 5: 157-161.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, University of München. http://edoc.ub.uni-muenchen.de/archive/00003175/ [EcoRI: 510 bp, 255 bp; TaqI: 270 bp, 210 bp, 195 bp, 110 bp]

– Item listing –